Active CMOS array sensor for time-resolved fluorescence detection

George Patounakis; Kenneth L. Shepard; Rastislav Levicky

doi:10.1109/JSSC.2006.883316

Active CMOS array sensor for time-resolved fluorescence detection

George Patounakis, Kenneth L. Shepard, Rastislav Levicky

Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Surface-based sensing assays based on fluorescence-based detection have become commonplace for both environmental and biomedical diagnostics. Traditional array scanners are expensive, large, and complex instruments. This paper describes the design of an active CMOS biosensor substrate for fluorescence-based assays that enables time-gated, time-resolved fluorescence spectroscopy without the need for an external reader. The array is sensitive to photon densities as low as 1.15 x 10s/cm3, has a dynamic range of over 74 dB, and has subnanosecond timing resolution. Sensitivity is achieved through subsampling and averaging.

Original language	English (US)
Article number	1717675
Pages (from-to)	2521-2530
Number of pages	10
Journal	IEEE Journal of Solid-State Circuits
Volume	41
Issue number	11
DOIs	https://doi.org/10.1109/JSSC.2006.883316
State	Published - Nov 2006

Keywords

Biochips
Biosensor
CMOS imager
Fluorescence spectroscopy
Microarray

ASJC Scopus subject areas

Electrical and Electronic Engineering

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10.1109/JSSC.2006.883316

Cite this

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title = "Active CMOS array sensor for time-resolved fluorescence detection",

abstract = "Surface-based sensing assays based on fluorescence-based detection have become commonplace for both environmental and biomedical diagnostics. Traditional array scanners are expensive, large, and complex instruments. This paper describes the design of an active CMOS biosensor substrate for fluorescence-based assays that enables time-gated, time-resolved fluorescence spectroscopy without the need for an external reader. The array is sensitive to photon densities as low as 1.15 x 10s/cm3, has a dynamic range of over 74 dB, and has subnanosecond timing resolution. Sensitivity is achieved through subsampling and averaging.",

keywords = "Biochips, Biosensor, CMOS imager, Fluorescence spectroscopy, Microarray",

author = "George Patounakis and Shepard, {Kenneth L.} and Rastislav Levicky",

note = "Funding Information: Manuscript received July 21, 2005; revised June 30, 2006. This work was supported in part by the National Institutes of Health under Grant HG003089 and by the National Science Foundation under Grant BES-0428544. Funding Information: Dr. Patounakis was the recipient of the Columbia SEAS Presidential Fellowship and an Intel Ph.D. fellowship.",

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doi = "10.1109/JSSC.2006.883316",

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AU - Shepard, Kenneth L.

AU - Levicky, Rastislav

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PY - 2006/11

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N2 - Surface-based sensing assays based on fluorescence-based detection have become commonplace for both environmental and biomedical diagnostics. Traditional array scanners are expensive, large, and complex instruments. This paper describes the design of an active CMOS biosensor substrate for fluorescence-based assays that enables time-gated, time-resolved fluorescence spectroscopy without the need for an external reader. The array is sensitive to photon densities as low as 1.15 x 10s/cm3, has a dynamic range of over 74 dB, and has subnanosecond timing resolution. Sensitivity is achieved through subsampling and averaging.

AB - Surface-based sensing assays based on fluorescence-based detection have become commonplace for both environmental and biomedical diagnostics. Traditional array scanners are expensive, large, and complex instruments. This paper describes the design of an active CMOS biosensor substrate for fluorescence-based assays that enables time-gated, time-resolved fluorescence spectroscopy without the need for an external reader. The array is sensitive to photon densities as low as 1.15 x 10s/cm3, has a dynamic range of over 74 dB, and has subnanosecond timing resolution. Sensitivity is achieved through subsampling and averaging.

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Active CMOS array sensor for time-resolved fluorescence detection

Abstract

Keywords

ASJC Scopus subject areas

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