Activity of T-type calcium channels is independent of CRMP2 in sensory neurons

Song Cai; Zhiming Shan; Zhongjun Zhang; Aubin Moutal; Rajesh Khanna

doi:10.1080/19336950.2019.1608129

Activity of T-type calcium channels is independent of CRMP2 in sensory neurons

Song Cai, Zhiming Shan, Zhongjun Zhang, Aubin Moutal, Rajesh Khanna

Research output: Contribution to journal › Article › peer-review

Abstract

Amongst the regulators of voltage-gated ion channels is the collapsin response mediator protein 2 (CRMP2). CRMP2 regulation of the activity and trafficking of NaV1.7 voltage-gated sodium channels as well as the N-type (CaV2.2) voltage-gated calcium channel (VGCC) has been reported. On the other hand, CRMP2 does not appear to regulate L- (CaV1.x), P/Q- (CaV2.1), and R- (CaV2.3) type high VGCCs. Whether CRMP2 regulates low VGCCs remains an open question. Here, we asked if CRMP2 could regulate the low voltage-gated (T-type/CaV3.x) channels in sensory neurons. Reducing CRMP2 protein levels with short interfering RNAs yielded no change in macroscopic currents carried by T-type channels. No change in biophysical properties of the T-type currents was noted. Future studies pursuing CRMP2 druggability in neuropathic pain will benefit from the findings that CRMP2 regulates only the N-type (CaV2.2) calcium channels.

Original language	English (US)
Pages (from-to)	147-152
Number of pages	6
Journal	Channels
Volume	13
Issue number	1
DOIs	https://doi.org/10.1080/19336950.2019.1608129
State	Published - Jan 1 2019

Keywords

CRMP2
DRG sensory neuron
T-type calcium channel
electrophysiology

ASJC Scopus subject areas

Biophysics
Biochemistry

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10.1080/19336950.2019.1608129

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title = "Activity of T-type calcium channels is independent of CRMP2 in sensory neurons",

abstract = "Amongst the regulators of voltage-gated ion channels is the collapsin response mediator protein 2 (CRMP2). CRMP2 regulation of the activity and trafficking of NaV1.7 voltage-gated sodium channels as well as the N-type (CaV2.2) voltage-gated calcium channel (VGCC) has been reported. On the other hand, CRMP2 does not appear to regulate L- (CaV1.x), P/Q- (CaV2.1), and R- (CaV2.3) type high VGCCs. Whether CRMP2 regulates low VGCCs remains an open question. Here, we asked if CRMP2 could regulate the low voltage-gated (T-type/CaV3.x) channels in sensory neurons. Reducing CRMP2 protein levels with short interfering RNAs yielded no change in macroscopic currents carried by T-type channels. No change in biophysical properties of the T-type currents was noted. Future studies pursuing CRMP2 druggability in neuropathic pain will benefit from the findings that CRMP2 regulates only the N-type (CaV2.2) calcium channels.",

keywords = "CRMP2, DRG sensory neuron, T-type calcium channel, electrophysiology",

author = "Song Cai and Zhiming Shan and Zhongjun Zhang and Aubin Moutal and Rajesh Khanna",

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doi = "10.1080/19336950.2019.1608129",

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AU - Cai, Song

AU - Shan, Zhiming

AU - Zhang, Zhongjun

AU - Moutal, Aubin

AU - Khanna, Rajesh

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Amongst the regulators of voltage-gated ion channels is the collapsin response mediator protein 2 (CRMP2). CRMP2 regulation of the activity and trafficking of NaV1.7 voltage-gated sodium channels as well as the N-type (CaV2.2) voltage-gated calcium channel (VGCC) has been reported. On the other hand, CRMP2 does not appear to regulate L- (CaV1.x), P/Q- (CaV2.1), and R- (CaV2.3) type high VGCCs. Whether CRMP2 regulates low VGCCs remains an open question. Here, we asked if CRMP2 could regulate the low voltage-gated (T-type/CaV3.x) channels in sensory neurons. Reducing CRMP2 protein levels with short interfering RNAs yielded no change in macroscopic currents carried by T-type channels. No change in biophysical properties of the T-type currents was noted. Future studies pursuing CRMP2 druggability in neuropathic pain will benefit from the findings that CRMP2 regulates only the N-type (CaV2.2) calcium channels.

AB - Amongst the regulators of voltage-gated ion channels is the collapsin response mediator protein 2 (CRMP2). CRMP2 regulation of the activity and trafficking of NaV1.7 voltage-gated sodium channels as well as the N-type (CaV2.2) voltage-gated calcium channel (VGCC) has been reported. On the other hand, CRMP2 does not appear to regulate L- (CaV1.x), P/Q- (CaV2.1), and R- (CaV2.3) type high VGCCs. Whether CRMP2 regulates low VGCCs remains an open question. Here, we asked if CRMP2 could regulate the low voltage-gated (T-type/CaV3.x) channels in sensory neurons. Reducing CRMP2 protein levels with short interfering RNAs yielded no change in macroscopic currents carried by T-type channels. No change in biophysical properties of the T-type currents was noted. Future studies pursuing CRMP2 druggability in neuropathic pain will benefit from the findings that CRMP2 regulates only the N-type (CaV2.2) calcium channels.

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KW - T-type calcium channel

KW - electrophysiology

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Activity of T-type calcium channels is independent of CRMP2 in sensory neurons

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