TY - JOUR
T1 - Activity of the mitochondrial multiple conductance channel is independent of the adenine nucleotide translocator
AU - Lohret, Timothy A.
AU - Murphy, Robert C.
AU - Drgoñ, Tomas
AU - Kinnally, Kathleen W.
PY - 1996/3/1
Y1 - 1996/3/1
N2 - The functional relationship between the adenine nucleotide translocator (ANT) and the mitochondrial multiple conductance channel (MCC) was investigated using patch-clamp techniques. MCC activity with the same conductance, ion selectivity, voltage dependence, and peptide sensitivity could be reconstituted from inner membrane fractions derived from mitochondria of ANT-deficient and wild-type Saccharomyces cerevisiae. In addition, the MCC activity of mouse kidney mitoplasts was unaffected by carboxyatractyloside, a known inhibitor of ANT and inducer of a permeability transition. These results suggest that MCC activity is independent of ANT.
AB - The functional relationship between the adenine nucleotide translocator (ANT) and the mitochondrial multiple conductance channel (MCC) was investigated using patch-clamp techniques. MCC activity with the same conductance, ion selectivity, voltage dependence, and peptide sensitivity could be reconstituted from inner membrane fractions derived from mitochondria of ANT-deficient and wild-type Saccharomyces cerevisiae. In addition, the MCC activity of mouse kidney mitoplasts was unaffected by carboxyatractyloside, a known inhibitor of ANT and inducer of a permeability transition. These results suggest that MCC activity is independent of ANT.
UR - http://www.scopus.com/inward/record.url?scp=0029981386&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029981386&partnerID=8YFLogxK
U2 - 10.1074/jbc.271.9.4846
DO - 10.1074/jbc.271.9.4846
M3 - Article
C2 - 8617754
AN - SCOPUS:0029981386
SN - 0021-9258
VL - 271
SP - 4846
EP - 4849
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 9
ER -