Adhesion strength-based, label-free isolation of human pluripotent stem cells

Ankur Singh; Shalu Suri; Ted Lee; Jamie M. Chilton; Marissa T. Cooke; Weiqiang Chen; Jianping Fu; Steven L. Stice; Hang Lu; Todd C. McDevitt; Andrés J. García

doi:10.1038/nmeth.2437

Adhesion strength-based, label-free isolation of human pluripotent stem cells

Ankur Singh, Shalu Suri, Ted Lee, Jamie M. Chilton, Marissa T. Cooke, Weiqiang Chen, Jianping Fu, Steven L. Stice, Hang Lu, Todd C. McDevitt, Andrés J. García

Research output: Contribution to journal › Article › peer-review

Abstract

We demonstrate substantial differences in 'adhesive signature' between human pluripotent stem cells (hPSCs), partially reprogrammed cells, somatic cells and hPSC-derived differentiated progeny. We exploited these differential adhesion strengths to rapidly (over ∼10 min) and efficiently isolate fully reprogrammed induced hPSCs (hiPSCs) as intact colonies from heterogeneous reprogramming cultures and from differentiated progeny using microfluidics. hiPSCs were isolated label free, enriched to 95%-99% purity with >80% survival, and had normal transcriptional profiles, differentiation potential and karyotypes. We also applied this strategy to isolate hPSCs (hiPSCs and human embryonic stem cells) during routine culture and show that it may be extended to isolate hPSC-derived lineage-specific stem cells or differentiated cells.

Original language	English (US)
Pages (from-to)	438-444
Number of pages	7
Journal	Nature methods
Volume	10
Issue number	5
DOIs	https://doi.org/10.1038/nmeth.2437
State	Published - May 2013

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.2437

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abstract = "We demonstrate substantial differences in 'adhesive signature' between human pluripotent stem cells (hPSCs), partially reprogrammed cells, somatic cells and hPSC-derived differentiated progeny. We exploited these differential adhesion strengths to rapidly (over ∼10 min) and efficiently isolate fully reprogrammed induced hPSCs (hiPSCs) as intact colonies from heterogeneous reprogramming cultures and from differentiated progeny using microfluidics. hiPSCs were isolated label free, enriched to 95%-99% purity with >80% survival, and had normal transcriptional profiles, differentiation potential and karyotypes. We also applied this strategy to isolate hPSCs (hiPSCs and human embryonic stem cells) during routine culture and show that it may be extended to isolate hPSC-derived lineage-specific stem cells or differentiated cells.",

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T1 - Adhesion strength-based, label-free isolation of human pluripotent stem cells

AU - Singh, Ankur

AU - Suri, Shalu

AU - Lee, Ted

AU - Chilton, Jamie M.

AU - Cooke, Marissa T.

AU - Chen, Weiqiang

AU - Fu, Jianping

AU - Stice, Steven L.

AU - Lu, Hang

AU - McDevitt, Todd C.

AU - García, Andrés J.

PY - 2013/5

Y1 - 2013/5

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AB - We demonstrate substantial differences in 'adhesive signature' between human pluripotent stem cells (hPSCs), partially reprogrammed cells, somatic cells and hPSC-derived differentiated progeny. We exploited these differential adhesion strengths to rapidly (over ∼10 min) and efficiently isolate fully reprogrammed induced hPSCs (hiPSCs) as intact colonies from heterogeneous reprogramming cultures and from differentiated progeny using microfluidics. hiPSCs were isolated label free, enriched to 95%-99% purity with >80% survival, and had normal transcriptional profiles, differentiation potential and karyotypes. We also applied this strategy to isolate hPSCs (hiPSCs and human embryonic stem cells) during routine culture and show that it may be extended to isolate hPSC-derived lineage-specific stem cells or differentiated cells.

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Adhesion strength-based, label-free isolation of human pluripotent stem cells

Abstract

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