Adhesion strength-based, label-free isolation of human pluripotent stem cells

Ankur Singh, Shalu Suri, Ted Lee, Jamie M. Chilton, Marissa T. Cooke, Weiqiang Chen, Jianping Fu, Steven L. Stice, Hang Lu, Todd C. McDevitt, Andrés J. García

Research output: Contribution to journalArticle

Abstract

We demonstrate substantial differences in 'adhesive signature' between human pluripotent stem cells (hPSCs), partially reprogrammed cells, somatic cells and hPSC-derived differentiated progeny. We exploited these differential adhesion strengths to rapidly (over ∼10 min) and efficiently isolate fully reprogrammed induced hPSCs (hiPSCs) as intact colonies from heterogeneous reprogramming cultures and from differentiated progeny using microfluidics. hiPSCs were isolated label free, enriched to 95%-99% purity with >80% survival, and had normal transcriptional profiles, differentiation potential and karyotypes. We also applied this strategy to isolate hPSCs (hiPSCs and human embryonic stem cells) during routine culture and show that it may be extended to isolate hPSC-derived lineage-specific stem cells or differentiated cells.

Original languageEnglish (US)
Pages (from-to)438-444
Number of pages7
JournalNature methods
Volume10
Issue number5
DOIs
StatePublished - May 2013

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Singh, A., Suri, S., Lee, T., Chilton, J. M., Cooke, M. T., Chen, W., Fu, J., Stice, S. L., Lu, H., McDevitt, T. C., & García, A. J. (2013). Adhesion strength-based, label-free isolation of human pluripotent stem cells. Nature methods, 10(5), 438-444. https://doi.org/10.1038/nmeth.2437