Advanced imaging for glaucoma study: Design, baseline characteristics, and inter-site comparison

Phuc V. Le, Xinbo Zhang, Brian A. Francis, Rohit Varma, David S. Greenfield, Joel S. Schuman, Nils Loewen, David Huang

Research output: Contribution to journalArticlepeer-review


Purpose To report the baseline characteristics of the participants in the Advanced Imaging for Glaucoma Study. To compare the participating sites for variations among subjects and the performance of imaging instruments. Design Multicenter longitudinal observational cohort study. Methods A total of 788 participants (1329 eyes) were enrolled from 3 academic referral centers. There were 145 participants (289 eyes) in the normal group, 394 participants (663 eyes) in the glaucoma suspect/preperimetric glaucoma group, and 249 participants (377 eyes) in the perimetric glaucoma group. Participants underwent a full clinical examination, standard automated perimetry, and imaging with time-domain and Fourier-domain optical coherence tomography (OCT), scanning laser polarimetry, and confocal scanning laser ophthalmoscopy. The baseline average, population standard deviation, and repeatability of imaging-derived anatomic variables were reported for each technology and center. Results Compared to the normal participants, glaucoma suspect/preperimetric glaucoma and perimetric glaucoma groups had significantly reduced anatomic measurements. Repeatability of nerve fiber layer thickness was best for Fourier-domain OCT (overall coefficient of variation <2%), followed by time-domain OCT (coefficient of variation 2%-2.9%), scanning laser polarimetry (coefficient of variation 2.6%-4.5%), and confocal scanning laser ophthalmoscopy rim area (coefficient of variation 4.2%-7.6%). A mixed-effects model showed that the differences between sites was less than 25 percent of the variation within groups and less than the differences between the normal and glaucoma suspect/preperimetric glaucoma group. Conclusions Site-to-site variation was smaller than both the variation within groups and the changes attributable to glaucoma. Therefore pooling of participants between sites is appropriate.

Original languageEnglish (US)
Pages (from-to)393-403.e2
JournalAmerican Journal of Ophthalmology
Issue number2
StatePublished - Feb 1 2015

ASJC Scopus subject areas

  • Ophthalmology


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