Advances and Opportunities in Single-Cell Transcriptomics for Plant Research

Carolin Seyfferth; Jim Renema; Jos R. Wendrich; Thomas Eekhout; Ruth Seurinck; Niels Vandamme; Bernhard Blob; Yvan Saeys; Yrjo Helariutta; Kenneth D. Birnbaum; Bert De Rybel

doi:10.1146/annurev-arplant-081720-010120

Advances and Opportunities in Single-Cell Transcriptomics for Plant Research

Carolin Seyfferth, Jim Renema, Jos R. Wendrich, Thomas Eekhout, Ruth Seurinck, Niels Vandamme, Bernhard Blob, Yvan Saeys, Yrjo Helariutta, Kenneth D. Birnbaum, Bert De Rybel

Biology and Genomics

Research output: Contribution to journal › Review article › peer-review

Abstract

Single-cell approaches are quickly changing our view on biological systems by increasing the spatiotemporal resolution of our analyses to the level of the individual cell. The field of plant biology has fully embraced single-cell transcriptomics and is rapidly expanding the portfolio of available technologies and applications. In this review, we give an overview of the main advances in plant single-cell transcriptomics over the past few years and provide the reader with an accessible guideline covering all steps, from sample preparation to data analysis. We end by offering a glimpse of how these technologies will shape and accelerate plant-specific research in the near future.

Original language	English (US)
Pages (from-to)	847-866
Number of pages	20
Journal	Annual Review of Plant Biology
Volume	72
DOIs	https://doi.org/10.1146/annurev-arplant-081720-010120
State	Published - Jun 17 2021

Keywords

cell atlas
cell trajectory
plant transcriptomics
protoplast
scRNA-seq

ASJC Scopus subject areas

Physiology
Molecular Biology
Plant Science
Cell Biology

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10.1146/annurev-arplant-081720-010120

Cite this

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title = "Advances and Opportunities in Single-Cell Transcriptomics for Plant Research",

abstract = "Single-cell approaches are quickly changing our view on biological systems by increasing the spatiotemporal resolution of our analyses to the level of the individual cell. The field of plant biology has fully embraced single-cell transcriptomics and is rapidly expanding the portfolio of available technologies and applications. In this review, we give an overview of the main advances in plant single-cell transcriptomics over the past few years and provide the reader with an accessible guideline covering all steps, from sample preparation to data analysis. We end by offering a glimpse of how these technologies will shape and accelerate plant-specific research in the near future.",

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T1 - Advances and Opportunities in Single-Cell Transcriptomics for Plant Research

AU - Seyfferth, Carolin

AU - Renema, Jim

AU - Wendrich, Jos R.

AU - Eekhout, Thomas

AU - Seurinck, Ruth

AU - Vandamme, Niels

AU - Blob, Bernhard

AU - Saeys, Yvan

AU - Helariutta, Yrjo

AU - Birnbaum, Kenneth D.

AU - De Rybel, Bert

PY - 2021/6/17

Y1 - 2021/6/17

N2 - Single-cell approaches are quickly changing our view on biological systems by increasing the spatiotemporal resolution of our analyses to the level of the individual cell. The field of plant biology has fully embraced single-cell transcriptomics and is rapidly expanding the portfolio of available technologies and applications. In this review, we give an overview of the main advances in plant single-cell transcriptomics over the past few years and provide the reader with an accessible guideline covering all steps, from sample preparation to data analysis. We end by offering a glimpse of how these technologies will shape and accelerate plant-specific research in the near future.

AB - Single-cell approaches are quickly changing our view on biological systems by increasing the spatiotemporal resolution of our analyses to the level of the individual cell. The field of plant biology has fully embraced single-cell transcriptomics and is rapidly expanding the portfolio of available technologies and applications. In this review, we give an overview of the main advances in plant single-cell transcriptomics over the past few years and provide the reader with an accessible guideline covering all steps, from sample preparation to data analysis. We end by offering a glimpse of how these technologies will shape and accelerate plant-specific research in the near future.

KW - cell atlas

KW - cell trajectory

KW - plant transcriptomics

KW - protoplast

KW - scRNA-seq

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DO - 10.1146/annurev-arplant-081720-010120

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JO - Annual Review of Plant Biology

JF - Annual Review of Plant Biology

ER -

Advances and Opportunities in Single-Cell Transcriptomics for Plant Research

Abstract

Keywords

ASJC Scopus subject areas

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