Air-plug enabled localization of single cell entrapment in an array of rectangular microwells

Pavithra Sukumar, Roaa Alnemari, Weiqiang Chen, Mohammad A. Qasaimeh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper demonstrates a microfluidic platform for central entrapment of single cell per well in an array of rectangular microwells, enabled by in situ air plugs formation. Reversible thermal bonding of two polydimethylsiloxane (PDMS) layers was shown to be sufficient in withstanding sequential flow of fluids at various flow rates. This work aim to isolate single T cells, within central regions of rectangular microwells, for multiplex cytokine detection.

Original languageEnglish (US)
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages671-672
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - Jan 1 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period10/22/1710/26/17

Keywords

  • Air-Plug Formation
  • Microfluidics
  • Microwells
  • PDMS
  • Single Cell
  • T Cell
  • Thermal Bonding

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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