Aldehyde capture ligation for synthesis of native peptide bonds

Monika Raj, Huabin Wu, Sarah L. Blosser, Marc A. Vittoria, Paramjit S. Arora

Research output: Contribution to journalArticle

Abstract

Chemoselective reactions for amide bond formation have transformed the ability to access synthetic proteins and other bioconjugates through ligation of fragments. In these ligations, amide bond formation is accelerated by transient enforcement of an intramolecular reaction between the carboxyl and the amine termini of two fragments. Building on this principle, we introduce an aldehyde capture ligation that parlays the high chemoselective reactivity of aldehydes and amines to enforce amide bond formation between amino acid residues and peptides that are difficult to ligate by existing technologies.

Original languageEnglish (US)
Pages (from-to)6932-6940
Number of pages9
JournalJournal of the American Chemical Society
Volume137
Issue number21
DOIs
StatePublished - Jun 3 2015

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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