Algorithm and benchmark dataset for stain separation in histology images

Michael T. McCann, Joshita Majumdar, Cheng Peng, Carlos A. Castro, Jelena Kovačević

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work, we present a new algorithm and benchmark dataset for stain separation in histology images. Histology is a critical and ubiquitous task in medical practice and research, serving as a gold standard of diagnosis for many diseases. Automating routine histology analysis tasks could reduce health care costs and improve diagnostic accuracy. One challenge in automation is that histology slides vary in their stain intensity and color; we therefore seek a digital method to normalize the appearance of histology images. As histology slides often have multiple stains on them that must be normalized independently, stain separation must occur before normalization. We propose a new digital stain separation method for the universally-used hematoxylin and eosin stain; this method improves on the state-of-the-art by adjusting the contrast of its eosin-only estimate and including a notion of stain interaction. To validate this method, we have collected a new benchmark dataset via chemical destaining containing ground truth images for stain separation, which we release publicly. Our experiments show that our method achieves more accurate stain separation than two comparison methods and that this improvement in separation accuracy leads to improved normalization.

Original languageEnglish (US)
Title of host publication2014 IEEE International Conference on Image Processing, ICIP 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages3953-3957
Number of pages5
ISBN (Electronic)9781479957514
DOIs
StatePublished - Jan 28 2014

Publication series

Name2014 IEEE International Conference on Image Processing, ICIP 2014

Keywords

  • histology
  • stain separation
  • unmixing

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition

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