TY - JOUR
T1 - Altered steady state and activity-dependent de novo protein expression in fragile X syndrome
AU - Bowling, Heather
AU - Bhattacharya, Aditi
AU - Zhang, Guoan
AU - Alam, Danyal
AU - Lebowitz, Joseph Z.
AU - Bohm-Levine, Nathaniel
AU - Lin, Derek
AU - Singha, Priyangvada
AU - Mamcarz, Maggie
AU - Puckett, Rosemary
AU - Zhou, Lili
AU - Aryal, Sameer
AU - Sharp, Kevin
AU - Kirshenbaum, Kent
AU - Berry-Kravis, Elizabeth
AU - Neubert, Thomas A.
AU - Klann, Eric
N1 - Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Whether fragile X mental retardation protein (FMRP) target mRNAs and neuronal activity contributing to elevated basal neuronal protein synthesis in fragile X syndrome (FXS) is unclear. Our proteomic experiments reveal that the de novo translational profile in FXS model mice is altered at steady state and in response to metabotropic glutamate receptor (mGluR) stimulation, but the proteins expressed differ under these conditions. Several altered proteins, including Hexokinase 1 and Ras, also are expressed in the blood of FXS model mice and pharmacological treatments previously reported to ameliorate phenotypes modify their abundance in blood. In addition, plasma levels of Hexokinase 1 and Ras differ between FXS patients and healthy volunteers. Our data suggest that brain-based de novo proteomics in FXS model mice can be used to find altered expression of proteins in blood that could serve as disease-state biomarkers in individuals with FXS.
AB - Whether fragile X mental retardation protein (FMRP) target mRNAs and neuronal activity contributing to elevated basal neuronal protein synthesis in fragile X syndrome (FXS) is unclear. Our proteomic experiments reveal that the de novo translational profile in FXS model mice is altered at steady state and in response to metabotropic glutamate receptor (mGluR) stimulation, but the proteins expressed differ under these conditions. Several altered proteins, including Hexokinase 1 and Ras, also are expressed in the blood of FXS model mice and pharmacological treatments previously reported to ameliorate phenotypes modify their abundance in blood. In addition, plasma levels of Hexokinase 1 and Ras differ between FXS patients and healthy volunteers. Our data suggest that brain-based de novo proteomics in FXS model mice can be used to find altered expression of proteins in blood that could serve as disease-state biomarkers in individuals with FXS.
UR - http://www.scopus.com/inward/record.url?scp=85064354382&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064354382&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-09553-8
DO - 10.1038/s41467-019-09553-8
M3 - Article
C2 - 30979884
AN - SCOPUS:85064354382
SN - 2041-1723
VL - 10
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 1710
ER -