Amygdala Kindling Alters Protein Kinase C Activity in Dentate Gyrus

Shu‐Jen ‐J Chen; Manisha A. Desai; Eric Klann; Danny G. Winder; J. David Sweatt; P. Jeffrey Conn

doi:10.1111/j.1471-4159.1992.tb11008.x

Amygdala Kindling Alters Protein Kinase C Activity in Dentate Gyrus

Shu‐Jen ‐J Chen, Manisha A. Desai, Eric Klann, Danny G. Winder, J. David Sweatt, P. Jeffrey Conn

Neural Science

Research output: Contribution to journal › Article › peer-review

Abstract

Abstract: Kindling is a use‐dependent form of synaptic plasticity and a widely used model of epilepsy. Although kindling has been widely studied, the molecular mechanisms underlying induction of this phenomenon are not well understood. We determined the effect of amygdala kindling on protein kinase C (PKC) activity in various regions of rat brain. Kindling stimulation markedly elevated basal (Ca²⁺‐independent) and Ca²⁺‐stimulated phosphorylation of an endogenous PKC substrate (which we have termed P17) in homogenates of dentate gyrus, assayed 2 h after kindling stimulation. The increase in P17 phosphorylation appeared to be due at least in part to persistent PKC activation, as basal PKC activity assayed in vitro using an exogenous peptide substrate was increased in kindled dentate gyrus 2 h after the last kindling stimulation. A similar increase in basal PKC activity was observed in dentate gyrus 2 h after the first kindling stimulation. These results document a kindling‐associated persistent PKC activation and suggest that the increased activity of PKC could play a role in the induction of the kindling effect.

Original language	English (US)
Pages (from-to)	1761-1769
Number of pages	9
Journal	Journal of Neurochemistry
Volume	59
Issue number	5
DOIs	https://doi.org/10.1111/j.1471-4159.1992.tb11008.x
State	Published - Nov 1992

Keywords

Dentate gyrus
Epilepsy model
Kindling
Protein kinase C activity

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1111/j.1471-4159.1992.tb11008.x

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title = "Amygdala Kindling Alters Protein Kinase C Activity in Dentate Gyrus",

abstract = "Abstract: Kindling is a use‐dependent form of synaptic plasticity and a widely used model of epilepsy. Although kindling has been widely studied, the molecular mechanisms underlying induction of this phenomenon are not well understood. We determined the effect of amygdala kindling on protein kinase C (PKC) activity in various regions of rat brain. Kindling stimulation markedly elevated basal (Ca2+‐independent) and Ca2+‐stimulated phosphorylation of an endogenous PKC substrate (which we have termed P17) in homogenates of dentate gyrus, assayed 2 h after kindling stimulation. The increase in P17 phosphorylation appeared to be due at least in part to persistent PKC activation, as basal PKC activity assayed in vitro using an exogenous peptide substrate was increased in kindled dentate gyrus 2 h after the last kindling stimulation. A similar increase in basal PKC activity was observed in dentate gyrus 2 h after the first kindling stimulation. These results document a kindling‐associated persistent PKC activation and suggest that the increased activity of PKC could play a role in the induction of the kindling effect.",

keywords = "Dentate gyrus, Epilepsy model, Kindling, Protein kinase C activity",

author = "Chen, {Shu‐Jen ‐J} and Desai, {Manisha A.} and Eric Klann and Winder, {Danny G.} and Sweatt, {J. David} and Conn, {P. Jeffrey}",

year = "1992",

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doi = "10.1111/j.1471-4159.1992.tb11008.x",

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AU - Chen, Shu‐Jen ‐J

AU - Desai, Manisha A.

AU - Klann, Eric

AU - Winder, Danny G.

AU - Sweatt, J. David

AU - Conn, P. Jeffrey

PY - 1992/11

Y1 - 1992/11

N2 - Abstract: Kindling is a use‐dependent form of synaptic plasticity and a widely used model of epilepsy. Although kindling has been widely studied, the molecular mechanisms underlying induction of this phenomenon are not well understood. We determined the effect of amygdala kindling on protein kinase C (PKC) activity in various regions of rat brain. Kindling stimulation markedly elevated basal (Ca2+‐independent) and Ca2+‐stimulated phosphorylation of an endogenous PKC substrate (which we have termed P17) in homogenates of dentate gyrus, assayed 2 h after kindling stimulation. The increase in P17 phosphorylation appeared to be due at least in part to persistent PKC activation, as basal PKC activity assayed in vitro using an exogenous peptide substrate was increased in kindled dentate gyrus 2 h after the last kindling stimulation. A similar increase in basal PKC activity was observed in dentate gyrus 2 h after the first kindling stimulation. These results document a kindling‐associated persistent PKC activation and suggest that the increased activity of PKC could play a role in the induction of the kindling effect.

AB - Abstract: Kindling is a use‐dependent form of synaptic plasticity and a widely used model of epilepsy. Although kindling has been widely studied, the molecular mechanisms underlying induction of this phenomenon are not well understood. We determined the effect of amygdala kindling on protein kinase C (PKC) activity in various regions of rat brain. Kindling stimulation markedly elevated basal (Ca2+‐independent) and Ca2+‐stimulated phosphorylation of an endogenous PKC substrate (which we have termed P17) in homogenates of dentate gyrus, assayed 2 h after kindling stimulation. The increase in P17 phosphorylation appeared to be due at least in part to persistent PKC activation, as basal PKC activity assayed in vitro using an exogenous peptide substrate was increased in kindled dentate gyrus 2 h after the last kindling stimulation. A similar increase in basal PKC activity was observed in dentate gyrus 2 h after the first kindling stimulation. These results document a kindling‐associated persistent PKC activation and suggest that the increased activity of PKC could play a role in the induction of the kindling effect.

KW - Dentate gyrus

KW - Epilepsy model

KW - Kindling

KW - Protein kinase C activity

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Amygdala Kindling Alters Protein Kinase C Activity in Dentate Gyrus

Abstract

Keywords

ASJC Scopus subject areas

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