Amyloid β-induced impairments in hippocampal synaptic plasticity are rescued by decreasing mitochondrial superoxide

Tao Ma, Charles A. Hoeffer, Helen Wong, Cynthia A. Massaad, Ping Zhou, Costantino Iadecola, Michael P. Murphy, Robia G. Pautler, Eric Klann

Research output: Contribution to journalArticlepeer-review


Generation of reactive oxygen species (ROS) causes cellular oxidative damage and has been implicated in the etiology of Alzheimer's disease (AD). In contrast, multiple lines of evidence indicate that ROS can normally modulate long-term potentiation (LTP), a cellular model for memory formation. We recently showed that decreasing the level of super oxide through the over expression of mitochondrial super oxide dismutase (SOD-2) prevents memory deficits in the Tg2576 mouse model of AD. In the current study, we explored whether AD-related LTP impairments could be prevented when ROS generation from mitochondria was diminished either pharmacologically or via genetic manipulation. In wild-type hippocampal slices treated with exogenous amyloid β peptide (Aβ1-42) and in slices from APP/PS1 mutant mice that model AD, LTP was impaired. The LTP impairments were prevented by MitoQ, a mitochondria-targeted antioxidant, and EUK134, an SOD and catalase mimetic. In contrast, inhibition of NADPH oxidase either by diphenyliodonium (DPI) or by genetically deleting gp91phox, the key enzymatic component of NADPH oxidase, had no effect on Aβ-induced LTP blockade. Moreover, live staining with MitoSOX Red, a mitochondrial super oxide indicator, combined with confocal microscopy, revealed that Aβ-induced super oxide production could be blunted by MitoQ, but not DPI, in agreement with our electrophysiological findings. Finally, in transgenic mice over expressing SOD-2, Aβ-induced LTP impairments and super oxide generation were prevented. Our data suggest a causal relationship between mitochondrial ROS imbalance and Aβ-induced impairments in hippocampal synaptic plasticity.

Original languageEnglish (US)
Pages (from-to)5589-5595
Number of pages7
JournalJournal of Neuroscience
Issue number15
StatePublished - Apr 13 2011

ASJC Scopus subject areas

  • General Neuroscience


Dive into the research topics of 'Amyloid β-induced impairments in hippocampal synaptic plasticity are rescued by decreasing mitochondrial superoxide'. Together they form a unique fingerprint.

Cite this