An actin-myosin complex on actively transcribing genes

Nathalie Fomproix; Piergiorgio Percipalle

doi:10.1016/j.yexcr.2003.10.028

An actin-myosin complex on actively transcribing genes

Nathalie Fomproix, Piergiorgio Percipalle

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Actin and myosin have been individually implicated in different aspects of gene expression. Here, we show in vivo evidence for a specific nucleolar actin-myosin complex physically associated with both the RNA polymerase I holoenzyme and ribosomal genes. We find that this specific actin-myosin complex is functionally coupled to elongating ribosomal RNA transcripts in living cells. From these observations, we conclude that an actin-based myosin motor is associated with transcribing ribosomal genes in the cell nucleus. These results correlate with an involvement of both actin and myosin in regulating mRNA synthesis and suggest that actin-myosin motors may provide a general mechanism to facilitate elongation of RNA transcripts during transcription of both ribosomal genes and protein-coding genes.

Original language	English (US)
Pages (from-to)	140-148
Number of pages	9
Journal	Experimental Cell Research
Volume	294
Issue number	1
DOIs	https://doi.org/10.1016/j.yexcr.2003.10.028
State	Published - Mar 10 2004

Keywords

Actin-myosin complex
Intranuclear motors
RNA elongation
RNA polymerase I
Ribosomal genes
Transcription

ASJC Scopus subject areas

Cell Biology

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10.1016/j.yexcr.2003.10.028

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title = "An actin-myosin complex on actively transcribing genes",

abstract = "Actin and myosin have been individually implicated in different aspects of gene expression. Here, we show in vivo evidence for a specific nucleolar actin-myosin complex physically associated with both the RNA polymerase I holoenzyme and ribosomal genes. We find that this specific actin-myosin complex is functionally coupled to elongating ribosomal RNA transcripts in living cells. From these observations, we conclude that an actin-based myosin motor is associated with transcribing ribosomal genes in the cell nucleus. These results correlate with an involvement of both actin and myosin in regulating mRNA synthesis and suggest that actin-myosin motors may provide a general mechanism to facilitate elongation of RNA transcripts during transcription of both ribosomal genes and protein-coding genes.",

keywords = "Actin-myosin complex, Intranuclear motors, RNA elongation, RNA polymerase I, Ribosomal genes, Transcription",

author = "Nathalie Fomproix and Piergiorgio Percipalle",

note = "Funding Information: We thank Bertil Daneholt, Neus Visa and David Bear for critically reading this manuscript. This work was supported by research grants from the Swedish Research Council (Vetenskapsr{\aa}det), the Lars Hiertas Minne Foundation and the Jeanssons Foundation to PP. NF was supported by a Marie Curie post-doctoral fellowship from the EC. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2004",

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doi = "10.1016/j.yexcr.2003.10.028",

language = "English (US)",

volume = "294",

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T1 - An actin-myosin complex on actively transcribing genes

AU - Fomproix, Nathalie

AU - Percipalle, Piergiorgio

N1 - Funding Information: We thank Bertil Daneholt, Neus Visa and David Bear for critically reading this manuscript. This work was supported by research grants from the Swedish Research Council (Vetenskapsrådet), the Lars Hiertas Minne Foundation and the Jeanssons Foundation to PP. NF was supported by a Marie Curie post-doctoral fellowship from the EC. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2004/3/10

Y1 - 2004/3/10

N2 - Actin and myosin have been individually implicated in different aspects of gene expression. Here, we show in vivo evidence for a specific nucleolar actin-myosin complex physically associated with both the RNA polymerase I holoenzyme and ribosomal genes. We find that this specific actin-myosin complex is functionally coupled to elongating ribosomal RNA transcripts in living cells. From these observations, we conclude that an actin-based myosin motor is associated with transcribing ribosomal genes in the cell nucleus. These results correlate with an involvement of both actin and myosin in regulating mRNA synthesis and suggest that actin-myosin motors may provide a general mechanism to facilitate elongation of RNA transcripts during transcription of both ribosomal genes and protein-coding genes.

AB - Actin and myosin have been individually implicated in different aspects of gene expression. Here, we show in vivo evidence for a specific nucleolar actin-myosin complex physically associated with both the RNA polymerase I holoenzyme and ribosomal genes. We find that this specific actin-myosin complex is functionally coupled to elongating ribosomal RNA transcripts in living cells. From these observations, we conclude that an actin-based myosin motor is associated with transcribing ribosomal genes in the cell nucleus. These results correlate with an involvement of both actin and myosin in regulating mRNA synthesis and suggest that actin-myosin motors may provide a general mechanism to facilitate elongation of RNA transcripts during transcription of both ribosomal genes and protein-coding genes.

KW - Actin-myosin complex

KW - Intranuclear motors

KW - RNA elongation

KW - RNA polymerase I

KW - Ribosomal genes

KW - Transcription

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DO - 10.1016/j.yexcr.2003.10.028

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AN - SCOPUS:1242316973

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SP - 140

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JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 1

ER -

An actin-myosin complex on actively transcribing genes

Abstract

Keywords

ASJC Scopus subject areas

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