An application of atomic force microscopy to measure the spring constant of single antigen-antibody complex

L. A. Chtcheglova, G. T. Shubeita, S. K. Sekatskii, G. Dietler

Research output: Contribution to journalArticle

Abstract

The aim of our study is to use Atomic Force Microscopy (AFM) to measure the spring constant for single bovine serum albumin (BSA) - polyclonal antibody to BSA (Ab-BSA) complex (kcomplex) as a function of pulling off force and complex extension. By the combination of AFM operated in the force-spectroscopy mode with the small dithering of the AFM tip we are able to detect the change of the dithering amplitude upon the pulling off process and to derive the value of kcomplex. At the moment of single specific bond rupture the spring constant value of 0.017 ± 0.003 N/m is determined.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalPhysics of Low-Dimensional Structures
Volume3-4
StatePublished - 2003

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Physics and Astronomy (miscellaneous)

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