An electrochemiluminescence assay for gp340 (DMBT1)

Cheryl Barber; Drew Weissman; Kurt Barnhart; Minal Dalvi; William R. Abrams; Daniel Malamud

doi:10.1016/j.ab.2013.05.015

An electrochemiluminescence assay for gp340 (DMBT1)

Cheryl Barber, Drew Weissman, Kurt Barnhart, Minal Dalvi, William R. Abrams, Daniel Malamud

Research output: Contribution to journal › Article › peer-review

Abstract

Gp340 is a member of the scavenger receptor cysteine-rich family of innate immune molecules and also functions as a tumor suppressor. This study describes a picogram-level assay using electrochemiluminescence technology on the MesoScale Discovery platform. Antibodies were evaluated and the best pair was used to assay whole-mouth stimulated saliva and cervical/vaginal lavage. The assay was tested using specimens obtained from healthy volunteers to determine if gp340 concentration in saliva correlates with levels in vaginal lavage fluid. Interestingly, no correlation was determined between gp340 content in these two fluids.

Original language	English (US)
Pages (from-to)	78-80
Number of pages	3
Journal	Analytical Biochemistry
Volume	440
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2013.05.015
State	Published - 2013

Keywords

Cervical vaginal lavage
MesoScale Discovery
Salivary agglutinin
Scavenger receptor cysteine-rich
Whole-mouth stimulated saliva

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.ab.2013.05.015

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title = "An electrochemiluminescence assay for gp340 (DMBT1)",

abstract = "Gp340 is a member of the scavenger receptor cysteine-rich family of innate immune molecules and also functions as a tumor suppressor. This study describes a picogram-level assay using electrochemiluminescence technology on the MesoScale Discovery platform. Antibodies were evaluated and the best pair was used to assay whole-mouth stimulated saliva and cervical/vaginal lavage. The assay was tested using specimens obtained from healthy volunteers to determine if gp340 concentration in saliva correlates with levels in vaginal lavage fluid. Interestingly, no correlation was determined between gp340 content in these two fluids.",

keywords = "Cervical vaginal lavage, MesoScale Discovery, Salivary agglutinin, Scavenger receptor cysteine-rich, Whole-mouth stimulated saliva",

author = "Cheryl Barber and Drew Weissman and Kurt Barnhart and Minal Dalvi and Abrams, {William R.} and Daniel Malamud",

note = "Funding Information: The support of the National Institute of Dental and Craniofacial Research ( U19DE018385 to D.M.) and the National Institute of Allergy and Infectious Disease ( AI094599-01 to D.M., AI082701 to D.W.) is gratefully acknowledged. D.M. is an NYStar recipient.",

year = "2013",

doi = "10.1016/j.ab.2013.05.015",

language = "English (US)",

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pages = "78--80",

journal = "Analytical Biochemistry",

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T1 - An electrochemiluminescence assay for gp340 (DMBT1)

AU - Barber, Cheryl

AU - Weissman, Drew

AU - Barnhart, Kurt

AU - Dalvi, Minal

AU - Abrams, William R.

AU - Malamud, Daniel

N1 - Funding Information: The support of the National Institute of Dental and Craniofacial Research ( U19DE018385 to D.M.) and the National Institute of Allergy and Infectious Disease ( AI094599-01 to D.M., AI082701 to D.W.) is gratefully acknowledged. D.M. is an NYStar recipient.

PY - 2013

Y1 - 2013

N2 - Gp340 is a member of the scavenger receptor cysteine-rich family of innate immune molecules and also functions as a tumor suppressor. This study describes a picogram-level assay using electrochemiluminescence technology on the MesoScale Discovery platform. Antibodies were evaluated and the best pair was used to assay whole-mouth stimulated saliva and cervical/vaginal lavage. The assay was tested using specimens obtained from healthy volunteers to determine if gp340 concentration in saliva correlates with levels in vaginal lavage fluid. Interestingly, no correlation was determined between gp340 content in these two fluids.

AB - Gp340 is a member of the scavenger receptor cysteine-rich family of innate immune molecules and also functions as a tumor suppressor. This study describes a picogram-level assay using electrochemiluminescence technology on the MesoScale Discovery platform. Antibodies were evaluated and the best pair was used to assay whole-mouth stimulated saliva and cervical/vaginal lavage. The assay was tested using specimens obtained from healthy volunteers to determine if gp340 concentration in saliva correlates with levels in vaginal lavage fluid. Interestingly, no correlation was determined between gp340 content in these two fluids.

KW - Cervical vaginal lavage

KW - MesoScale Discovery

KW - Salivary agglutinin

KW - Scavenger receptor cysteine-rich

KW - Whole-mouth stimulated saliva

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An electrochemiluminescence assay for gp340 (DMBT1)

Abstract

Keywords

ASJC Scopus subject areas

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