An evaluation of detection methods for large lariat RNAs

Candice E. Coombes, Jef D. Boeke

    Research output: Contribution to journalArticle

    Abstract

    Ty1 elements are long terminal repeat (LTR) retrotransposons that reside within the genome of Saccharomyces cerevisiae. It has been known for many years that the 2′-5′ phosphodiesterase Dbr1p, which debranches intron lariats, is required for efficient Ty1 transposition. A recent report suggested the intriguing possibility that Ty1 RNA forms a lariat as a transposition intermediate. We set out to further investigate the nature of the proposed Ty1 lariat branchpoint. However, using a wide range of techniques we were unable to find any evidence for the proposed lariat structure. Furthermore, we demonstrate that some of the techniques used in the initial study describing the lariat are capable of incorrectly reporting a lariat structure. Thus, the role of the Dbr1 protein in Ty1 retrotransposition remains elusive.

    Original languageEnglish (US)
    Pages (from-to)323-331
    Number of pages9
    JournalRNA
    Volume11
    Issue number3
    DOIs
    StatePublished - Mar 2005

    Keywords

    • Lariat
    • RNase protection
    • Retrotransposition
    • Ty1

    ASJC Scopus subject areas

    • Molecular Biology

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