An HIV type 2 DNA vaccine induces cross-reactive immune responses against HIV type 2 and SIV

M. G. Agadjanyan, N. N. Trivedi, S. Kudchodkar, M. Bennett, W. Levine, A. Lin, J. Boyer, D. Levy, K. E. Ugen, J. J. Kim, D. B. Weiner

Research output: Contribution to journalArticle

Abstract

We have previously reported on the generation of specific functional immune responses after inoculation of animals with expression vectors encoding HIV-1 genes. This article provides the details of the first application of this new technology to induce immune responses against HIV-2. This virus is molecularly and serologically distinct from HIV-1 and is in fact more closely related to the simian immunodeficiency virus (SIV). Anti- HIV-2 and SIV antibodies were induced in mice of three different haplotypes following a single intramuscular inoculation with an HIV-2/ROD envelope glycoprotein expression vector (pcEnv-2). Boosting of animals with pcEnv-2 induced both anti-HIV-2 neutralizing antibodies and T cell-proliferative responses against HIV-2 and SIVmac proteins. We compared the humoral and cellular immune responses of mice injected with pcEnv-2 and then boosted with either the homologous DNA construct or a recombinant Env protein. Animals boosted with pcEnv-2 generated B and T cell immune responses as strong as those of mice boosted with recombinant gp140 protein in adjuvant. Finally, cellular immune responses were significantly increased with the coadministration of pcEnv-2 and a plasmid expressing interleukin 12. We therefore conclude that DNA plasmid inoculation induces cross-reactive anti- HIV-2 and anti-SIVmac immune responses in mice. This technology should be further investigated as a potential vaccine component for this human pathogen.

Original languageEnglish (US)
Pages (from-to)1561-1572
Number of pages12
JournalAIDS Research and Human Retroviruses
Volume13
Issue number18
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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    Agadjanyan, M. G., Trivedi, N. N., Kudchodkar, S., Bennett, M., Levine, W., Lin, A., Boyer, J., Levy, D., Ugen, K. E., Kim, J. J., & Weiner, D. B. (1997). An HIV type 2 DNA vaccine induces cross-reactive immune responses against HIV type 2 and SIV. AIDS Research and Human Retroviruses, 13(18), 1561-1572. https://doi.org/10.1089/aid.1997.13.1561