An immobile nucleic acid junction constructed from oligonucleotides

Neville R. Kallenbach, Rong Ine Ma, Nadrian C. Seeman

Research output: Contribution to journalArticlepeer-review

Abstract

Base-paired DNA duplexes involving oligonucleotide model systems have provided the major source of detailed structural and dynamic information about double helical structure1. Triple- and quadruple-branched 'junction' structures of DNA have a transient existence as intermediates in the replication or recombination of DNA molecules2-5 while cruciforms may be inducible by negatively supercoiling closed circular DNA6-11. However, it has not been possible to investigate these forms structurally at high resolution in short-chain molecules, where the junction will yield a significant component of the signal, because these naturally occurring intermediates are inherently unstable, due to internal sequence symmetry, which permits their resolution to double helices, via branchpoint migration 12-15. We have recently proposed that migration can be eliminated to yield immobile junctions from oligonucleotides16-19 by combining sequence symmetry constraints with equilibrium calculations. We present here electrophoretic and UV optical absorbance experiments which indicate that four hexadecadeoxynucleotides (Fig. 1) indeed do form a stable tetrameric junction complex in solution.

Original languageEnglish (US)
Pages (from-to)829-831
Number of pages3
JournalNature
Volume305
Issue number5937
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'An immobile nucleic acid junction constructed from oligonucleotides'. Together they form a unique fingerprint.

Cite this