@inproceedings{869ef84d2ecc4cf287256a5d76f08740,
title = "An integrated microfluidic platform for in-situ cellular cytokine secretion immunophenotyping",
abstract = "Rapid, quantitative detection of cell-secreted biomarker proteins with a low sample volume holds great promise to advance cellular immunophenotyping techniques for diagnosis and treatment of infectious diseases. Here we successfully developed an immunophenotyping assay with THP-1 human moncytic leukemia cells using a highly integrated microfluidic platform incorporating a no-wash bead-based chemiluminescence immunodetection scheme. Our study achieved ultra-sensitive cellular immunophenotyping with 20-fold fewer cells and 7 times faster than the conventional enzyme-linked immunosorbent assay (ELISA). Our strategy of monitoring immune cell functions could impact medical treatments of infectious diseases by enabling a sample-efficient cellular immunophenotyping analysis.",
keywords = "Cellular immunophenotyping, Chemiluminescence, Microfluidics, Optics",
author = "Huang, {Nien Tsu} and Weiqiang Chen and Oh, {Bo Ram} and Jianping Fu and Katsuo Kurabayashi",
year = "2012",
language = "English (US)",
isbn = "9780979806452",
series = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
publisher = "Chemical and Biological Microsystems Society",
pages = "989--991",
booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",
}