TY - GEN
T1 - AN INTEGRATED MICROFLUIDIC PROBE FOR CYTOPLASMIC BIOPSY OF SINGLE ADHERENT CELLS
AU - Sofela, Samuel
AU - Brimmo, Ayoola
AU - Qasaimeh, Mohammad A.
N1 - Funding Information:
This work was supported by NYU Abu Dhabi and ADEK Excellence Award (AARE19-187), Abu Dhabi, UAE.
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Single cell omics analysis has become the gold standard for understanding cellular mechanisms and heterogeneity with tissues, however, most available techniques depend on suspended cells where the spatiotemporal arrangement of cell heterogeneity within tissue is lost. In this report, we present an integrated microfluidic probe (MFP) that utilize the concepts of electropermealization (EP) and hydrodynamic flow confinement (HFC) to perform contactless single cell cytoplasmic biopsy on adherent cell culture, and potentially on tissue slices.
AB - Single cell omics analysis has become the gold standard for understanding cellular mechanisms and heterogeneity with tissues, however, most available techniques depend on suspended cells where the spatiotemporal arrangement of cell heterogeneity within tissue is lost. In this report, we present an integrated microfluidic probe (MFP) that utilize the concepts of electropermealization (EP) and hydrodynamic flow confinement (HFC) to perform contactless single cell cytoplasmic biopsy on adherent cell culture, and potentially on tissue slices.
KW - biopsy
KW - electropermealization
KW - microfluidic probe
KW - single cell
KW - Transcriptomics
UR - http://www.scopus.com/inward/record.url?scp=85136964420&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136964420&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136964420
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1073
EP - 1074
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -