@inbook{9577502ab8d84cbb972ee3aca5374c9a,
title = "Antibody pull-down experiments in fission yeast",
abstract = "Proteins act as executors for almost all kinds of cellular processes. The majority of proteins achieve their proper functions through interacting with other proteins. Knowing the binding partners of a protein is instrumental for understanding its function. The antibody pull-down method is a powerful and common approach to detect protein-protein interactions. Here, an antibody pull-down protocol is described for detecting protein-protein interactions in fission yeast Schizosaccharomyces pombe.",
keywords = "Antibody, Co-immunoprecipitation, Fission yeast, In vivo, Protein-protein interaction, Pull-down, Schizosaccharomyces pombe",
author = "Qianhua Dong and Fei Li",
note = "Funding Information: We would like to thank past and present members of Li lab who contributed to the methods presented here, and thank Dr. Jinpu Yang for reading the manuscript. F.L. is a Pew Scholar in the Biomedical Sciences, supported by The Pew Charitable Trusts. This work was supported by National Institutes of Health grant R01GM106037 (to F.L.)., National Science Foundation grant MCB-1330557 (to F.L.), and the National Natural Science Foundation of China (NSFC) Grant 31728010 (to F.L.).",
year = "2018",
doi = "10.1007/978-1-4939-7546-4_11",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "117--123",
booktitle = "Methods in Molecular Biology",
}