Antibody pull-down experiments in fission yeast

Qianhua Dong, Fei Li

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Proteins act as executors for almost all kinds of cellular processes. The majority of proteins achieve their proper functions through interacting with other proteins. Knowing the binding partners of a protein is instrumental for understanding its function. The antibody pull-down method is a powerful and common approach to detect protein-protein interactions. Here, an antibody pull-down protocol is described for detecting protein-protein interactions in fission yeast Schizosaccharomyces pombe.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages117-123
Number of pages7
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1721
ISSN (Print)1064-3745

Keywords

  • Antibody
  • Co-immunoprecipitation
  • Fission yeast
  • In vivo
  • Protein-protein interaction
  • Pull-down
  • Schizosaccharomyces pombe

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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