Apoptosis of growth plate chondrocytes occurs through a mitochondrial pathway

Cristina C. Teixeira, Aida P.Padron Costas, Yelena Nemelivsky

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To determine the role of mitochondria in chondrocyte apoptosis induced by inorganic phosphate (Pi). Materials and Methods: Chondrocytes isolated from the growth plates of chick embryo tibia were treated with Pi in serum-free media; chondrocyte viability, mitochondrial membrane potential, cytochrome c release from mitochondria, caspase 3 activity, endonuclease activity, and DNA fragmentation were investigated. Results: Exposure to Pi for 24 hours induced apoptosis in growth plate chondrocytes through a pathway that involved loss of mitochondrial function, release of cytochrome c into the cytoplasm, increases in caspase 3 and endonuclease activities, and fragmentation of DNA. Conclusions: This study suggests that mitochondria are important players in Pi-induced apoptosis.

Original languageEnglish (US)
Pages (from-to)129-134
Number of pages6
JournalAngle Orthodontist
Volume77
Issue number1
DOIs
StatePublished - Jan 2007

Keywords

  • Apoptosis
  • Caspases
  • Chondrocytes
  • Endonucleases
  • Growth plate
  • Inorganic phosphate

ASJC Scopus subject areas

  • Orthodontics

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