TY - JOUR
T1 - Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line
AU - Pardo-Mora, Dolly Patricia
AU - Murillo, Oscar Julián
AU - Rey-Buitrago, Mauricio
AU - Losada-Barragán, Mónica
AU - Uribe, Jaime Fabian Cruz
AU - Santiago, Karina Basso
AU - Conti, Bruno José
AU - de Oliveira Cardoso, Eliza
AU - Conte, Fernanda Lopes
AU - Gutiérrez, Rafael María
AU - García, Orlando Torres
AU - Sforcin, José Maurício
N1 - Publisher Copyright:
© 2021 Veterinary World. All rights reserved.
PY - 2021/4
Y1 - 2021/4
N2 - Background and Aim: Osteosarcoma (OSA) is the most common bone tumor in canines and humans. This study aimed to assess the cytotoxic and apoptotic effects of Colombian propolis samples on a canine OSA cell line (OSCA-8) by evaluating the expression of BCL-2, BAX, CASPASE 9, CASPASE 8, and TNFR1 genes involved in the apoptosis pathway. Materials and Methods: After treating the cells with five Colombian propolis samples (Usm, Met, Fus, Sil, and Caj), we evaluated cell viability and lactate dehydrogenase (LDH) release. Early and late apoptosis was determined by flow cytometry using annexin V/propidium iodide. Furthermore, the effects of three selected samples on gene expression were analyzed by real-time polymerase chain reaction. Results: The Colombian propolis samples reduced OSCA-8 cell viability and increased LDH release. All samples induced apoptosis significantly and upregulated BCL-2 and CASPASE 8 expression. Usm and Sil increased BAX expression, Met and Sil induced CASPASE 9 expression, and Usm increased TNFR1. Conclusion: Colombian propolis samples exhibited cytotoxic and apoptotic effects on canine OSA cells, and CASPASE 8 upregulation indicated apoptosis induction by the extrinsic pathway.
AB - Background and Aim: Osteosarcoma (OSA) is the most common bone tumor in canines and humans. This study aimed to assess the cytotoxic and apoptotic effects of Colombian propolis samples on a canine OSA cell line (OSCA-8) by evaluating the expression of BCL-2, BAX, CASPASE 9, CASPASE 8, and TNFR1 genes involved in the apoptosis pathway. Materials and Methods: After treating the cells with five Colombian propolis samples (Usm, Met, Fus, Sil, and Caj), we evaluated cell viability and lactate dehydrogenase (LDH) release. Early and late apoptosis was determined by flow cytometry using annexin V/propidium iodide. Furthermore, the effects of three selected samples on gene expression were analyzed by real-time polymerase chain reaction. Results: The Colombian propolis samples reduced OSCA-8 cell viability and increased LDH release. All samples induced apoptosis significantly and upregulated BCL-2 and CASPASE 8 expression. Usm and Sil increased BAX expression, Met and Sil induced CASPASE 9 expression, and Usm increased TNFR1. Conclusion: Colombian propolis samples exhibited cytotoxic and apoptotic effects on canine OSA cells, and CASPASE 8 upregulation indicated apoptosis induction by the extrinsic pathway.
KW - Apoptosis
KW - Cytotoxicity
KW - Osteosarcoma
KW - Propolis
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U2 - 10.14202/vetworld.2021.964-971
DO - 10.14202/vetworld.2021.964-971
M3 - Article
AN - SCOPUS:85105266911
SN - 0972-8988
VL - 14
SP - 964
EP - 971
JO - Veterinary World
JF - Veterinary World
IS - 4
ER -