Aqueous Synthesis of Triphenylphosphine-Modified Gold Nanoparticles for Synergistic In Vitro and In Vivo Photothermal Chemotherapy

Farah Benyettou, Anjana Ramdas Nair, Yaereen Dho, Thirumurugan Prakasam, Renu Pasricha, Jamie Whelan, Hassan Traboulsi, Javed Mazher, Kirsten C. Sadler, Ali Trabolsi

Research output: Contribution to journalArticlepeer-review

Abstract

Triphenylphosphine (TPP) surface-functionalized and F-108 Pluronic-stabilized gold nanoparticles (F-108@TPP-AuNPs) have been synthesized through a one-step approach, leading to well-defined (9.6±1.6 nm) and water-soluble nanoparticles by microwave heating an aqueous solution of TPP-AuICl in the presence of a Pluronic polymer under basic conditions. TPP release was negligible under physiological conditions, but enhanced significantly at an acidic pH (5.4) mimicking that of a cancer cell. Laser irradiation (532 nm) raised the temperature of an aqueous solution of F-108@TPP-AuNPs to 51.7 °C within 5 min, confirming efficient light-to-heat conversion capabilities without significant photodegradation. TEM confirmed intracellular localization of F-108@TPP-AuNPs in the cytosol, endosomes and lysosomes of HeLa cells. F-108@TPP-AuNPs were well tolerated by HeLa cells and zebrafish embryos at ambient temperatures and became toxic upon heat activation, suggesting synergistic interactions between heat and cytotoxic action by TPP.

Original languageEnglish (US)
Pages (from-to)5270-5279
Number of pages10
JournalChemistry - A European Journal
Volume26
Issue number23
DOIs
StatePublished - Apr 21 2020

Keywords

  • apoptosis
  • chemotherapy
  • drug delivery system
  • hyperthermia
  • zebrafish

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry

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