Ascorbic Acid Inhibition of Cyclic Nucleotide Phosphodiesterase Activity

Daniel Malamud; Yale Kroll

doi:10.3181/00379727-164-40911

Ascorbic Acid Inhibition of Cyclic Nucleotide Phosphodiesterase Activity

Daniel Malamud, Yale Kroll

Research output: Contribution to journal › Article › peer-review

Abstract

Low levels of ascorbic acid reversibly inhibit mouse brain and beef heart cyclic nucleotide phosphodiesterase activity in vitro. Inhibition is noncompetitive with a K_i = 10^-4 M. Similar inhibition occurs with D-ascorbate but not with dehydroascorbate or metabisulfite. The kinetics of inhibition by ascorbic acid were similar for “high” and “low” K_m cAMP phosphodiesterase and cGMP phosphodiesterase activity.

Original language	English (US)
Pages (from-to)	534-536
Number of pages	3
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	164
Issue number	4
DOIs	https://doi.org/10.3181/00379727-164-40911
State	Published - Sep 1980

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "Ascorbic Acid Inhibition of Cyclic Nucleotide Phosphodiesterase Activity",

abstract = "Low levels of ascorbic acid reversibly inhibit mouse brain and beef heart cyclic nucleotide phosphodiesterase activity in vitro. Inhibition is noncompetitive with a Ki = 10-4 M. Similar inhibition occurs with D-ascorbate but not with dehydroascorbate or metabisulfite. The kinetics of inhibition by ascorbic acid were similar for “high” and “low” Km cAMP phosphodiesterase and cGMP phosphodiesterase activity.",

author = "Daniel Malamud and Yale Kroll",

note = "Funding Information: This research was supported by N.I.H. Grant GM-21700.",

year = "1980",

month = sep,

doi = "10.3181/00379727-164-40911",

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journal = "Proceedings of the Society for Experimental Biology and Medicine",

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T1 - Ascorbic Acid Inhibition of Cyclic Nucleotide Phosphodiesterase Activity

AU - Malamud, Daniel

AU - Kroll, Yale

N1 - Funding Information: This research was supported by N.I.H. Grant GM-21700.

PY - 1980/9

Y1 - 1980/9

N2 - Low levels of ascorbic acid reversibly inhibit mouse brain and beef heart cyclic nucleotide phosphodiesterase activity in vitro. Inhibition is noncompetitive with a Ki = 10-4 M. Similar inhibition occurs with D-ascorbate but not with dehydroascorbate or metabisulfite. The kinetics of inhibition by ascorbic acid were similar for “high” and “low” Km cAMP phosphodiesterase and cGMP phosphodiesterase activity.

AB - Low levels of ascorbic acid reversibly inhibit mouse brain and beef heart cyclic nucleotide phosphodiesterase activity in vitro. Inhibition is noncompetitive with a Ki = 10-4 M. Similar inhibition occurs with D-ascorbate but not with dehydroascorbate or metabisulfite. The kinetics of inhibition by ascorbic acid were similar for “high” and “low” Km cAMP phosphodiesterase and cGMP phosphodiesterase activity.

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IS - 4

ER -

Ascorbic Acid Inhibition of Cyclic Nucleotide Phosphodiesterase Activity

Abstract

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