TY - JOUR
T1 - Assessment of the anti-microbial agent C31G as a spermicide
T2 - Comparison with nonoxynol-9
AU - Thompson, Kathleen A.
AU - Malamud, Daniel
AU - Bayard, Storey T.
N1 - Funding Information:
We acknowledge with gratitude the assistance of Susan Heyner, Ph.D., Director of the Andrology Laboratory, in providing semen samples, the assistance of Louise Borio, RN and Luigi Mastroianni, Jr., M.D., in providing the samples of cervical mucus. This work was supported by NIH contract NO 1-HD-3-3193 from NICHD.
PY - 1996/5
Y1 - 1996/5
N2 - The broad-spectrum anti-microbial agent, C31G, containing an equimolar mixture of n-dodecyl-dimethylamine-N-oxide (C12-N-O) and N (n-dodecyl), N- dimethyl-glycine (C12-betaine), was tested for spermicidal activity in comparison with the currently used spermicide, nonoxynol-9 (N-9). The rate of sperm cell permeabilization by the spermicides, as assayed with the fluorescent probe, TO-PRO-1, increased as the cube of the C31G concentration, while the rate increase was linear with N-9 concentration. At 0.04%, the rate of sperm cell permeabilization with both spermicides is at the limit of rapid measurement. C31G diffuses through cervical mucus at a more rapid rate than does N-9. C31G has long been known to aid wound healing and reduce inflammation, whereas N-9 has been reported to induce vaginal irritation. C31G would, thus, seem to have the spermicidal efficacy, the broad range of anti-microbial activity, and the lack of inflammatory activity that is sought in the ideal vaginal spermicide.
AB - The broad-spectrum anti-microbial agent, C31G, containing an equimolar mixture of n-dodecyl-dimethylamine-N-oxide (C12-N-O) and N (n-dodecyl), N- dimethyl-glycine (C12-betaine), was tested for spermicidal activity in comparison with the currently used spermicide, nonoxynol-9 (N-9). The rate of sperm cell permeabilization by the spermicides, as assayed with the fluorescent probe, TO-PRO-1, increased as the cube of the C31G concentration, while the rate increase was linear with N-9 concentration. At 0.04%, the rate of sperm cell permeabilization with both spermicides is at the limit of rapid measurement. C31G diffuses through cervical mucus at a more rapid rate than does N-9. C31G has long been known to aid wound healing and reduce inflammation, whereas N-9 has been reported to induce vaginal irritation. C31G would, thus, seem to have the spermicidal efficacy, the broad range of anti-microbial activity, and the lack of inflammatory activity that is sought in the ideal vaginal spermicide.
KW - C31G efficacy
KW - TO-PRO-1 cell lysis probe
KW - anti-microbial
KW - cervical mucus
KW - nonoxynol-9
KW - sperm permeabilization
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U2 - 10.1016/0010-7824(96)00066-2
DO - 10.1016/0010-7824(96)00066-2
M3 - Article
C2 - 8724622
AN - SCOPUS:0029894732
SN - 0010-7824
VL - 53
SP - 313
EP - 318
JO - Contraception
JF - Contraception
IS - 5
ER -