@article{8179639dd8ac451887553d04212eb563,
title = "Assignment of the human ferrochelatase gene (FECH) and a locus for protoporphyria to chromosome 18q22",
abstract = "We have mapped the human gene for ferrochelatase (FECH; ferroheme-protolyase, EC 4.99.1.1) to chromosome 18 by hybridization of cDNA to sorted chromosomes. The probe was obtained by PCR-directed amplification of a human marrow cDNA library in λgt 10. Subchromosomal localization of ferrochelatase to 18q22 was determined by chromosomal hybridization in situ using a human ferrochelatase genomic clone in λEMBL 3 that contained a 20-kb insert. Since ferrochelatase activity is deficient in patients with the inherited disease erythropoietic protoporphyria, a locus for this disease may be assigned to 18q22, one of few monogenic defects that have been mapped to this chromosome.",
author = "Whitcombe, {David M.} and Carter, {Nigel P.} and Albertson, {Donna G.} and Smith, {Stuart J.} and Rhodes, {David A.} and Cox, {Timothy M.}",
note = "Funding Information: Mrs. Rita Fishpool kindly assisted with the labeling of genomic probes for the in situ hybridization studies. Ms. Adele Pelmear assisted with the flow-sorted dot blot experiments. We also thank Dr. Robert Sarkany for informative discussions and Mrs. Joan Grantham for preparation of the manuscript. We are indebted to Dr. Rosine Labbe-Bois for making available the sequence of the HEM 15 gene in advance of publication. The work was supported by The Wellcome Trust and the Medical Research Council as part of the UK Human Genome Project.",
year = "1991",
month = dec,
doi = "10.1016/0888-7543(91)90044-F",
language = "English (US)",
volume = "11",
pages = "1152--1154",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "4",
}