Abstract
We have mapped the human gene for ferrochelatase (FECH; ferroheme-protolyase, EC 4.99.1.1) to chromosome 18 by hybridization of cDNA to sorted chromosomes. The probe was obtained by PCR-directed amplification of a human marrow cDNA library in λgt 10. Subchromosomal localization of ferrochelatase to 18q22 was determined by chromosomal hybridization in situ using a human ferrochelatase genomic clone in λEMBL 3 that contained a 20-kb insert. Since ferrochelatase activity is deficient in patients with the inherited disease erythropoietic protoporphyria, a locus for this disease may be assigned to 18q22, one of few monogenic defects that have been mapped to this chromosome.
Original language | English (US) |
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Pages (from-to) | 1152-1154 |
Number of pages | 3 |
Journal | Genomics |
Volume | 11 |
Issue number | 4 |
DOIs | |
State | Published - Dec 1991 |
ASJC Scopus subject areas
- Genetics