Asymmetric methyl group labeling as a probe of membrane protein homo-oligomers by NMR spectroscopy

Nathaniel J. Traaseth, Raffaello Verardi, Gianluigi Veglia

Research output: Contribution to journalArticlepeer-review

Abstract

We present an asymmetric isotopic labeling strategy for probing unambiguously membrane protein binding interfaces in homo-oligomers. This technique is highly sensitive, rapid, and requires only one 13C methyl-edited nuclear Overhauser effect spectroscopy (NOESY) spectrum. As a proof of principle, we apply this technique to the homo-pentameric membrane protein phospholamban, defining the leucine-isoleucine zipper interface between its monomers with atomistic resolution.

Original languageEnglish (US)
Pages (from-to)2400-2401
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number8
DOIs
StatePublished - Feb 27 2008

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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