AtNIGT1/HRS1 integrates nitrate and phosphate signals at the Arabidopsis root tip

Anna Medici; Amy Marshall-Colon; Elsa Ronzier; Wojciech Szponarski; Rongchen Wang; Alain Gojon; Nigel M. Crawford; Sandrine Ruffel; Gloria M. Coruzzi; Gabriel Krouk

doi:10.1038/ncomms7274

AtNIGT1/HRS1 integrates nitrate and phosphate signals at the Arabidopsis root tip

Anna Medici, Amy Marshall-Colon, Elsa Ronzier, Wojciech Szponarski, Rongchen Wang, Alain Gojon, Nigel M. Crawford, Sandrine Ruffel, Gloria M. Coruzzi, Gabriel Krouk

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

Nitrogen and phosphorus are among the most widely used fertilizers worldwide. Nitrate (NO₃^-) and phosphate (PO₄^3-) are also signalling molecules whose respective transduction pathways are being intensively studied. However, plants are continuously challenged with combined nutritional deficiencies, yet very little is known about how these signalling pathways are integrated. Here we report the identification of a highly NO₃^- -inducible NRT1.1-controlled GARP transcription factor, HRS1, document its genome-wide transcriptional targets, and validate its cis-regulatory elements. We demonstrate that this transcription factor and a close homologue repress the primary root growth in response to P deficiency conditions, but only when NO₃^- is present. This system defines a molecular logic gate integrating P and N signals. We propose that NO₃^- and P signalling converge via double transcriptional and post-transcriptional control of the same protein, HRS1.

Original language	English (US)
Article number	6274
Journal	Nature communications
Volume	6
DOIs	https://doi.org/10.1038/ncomms7274
State	Published - Feb 27 2015

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
General
Physics and Astronomy(all)

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10.1038/ncomms7274

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@article{78958989b44b4109bef2af2f627edc6e,

title = "AtNIGT1/HRS1 integrates nitrate and phosphate signals at the Arabidopsis root tip",

abstract = "Nitrogen and phosphorus are among the most widely used fertilizers worldwide. Nitrate (NO3-) and phosphate (PO43-) are also signalling molecules whose respective transduction pathways are being intensively studied. However, plants are continuously challenged with combined nutritional deficiencies, yet very little is known about how these signalling pathways are integrated. Here we report the identification of a highly NO3- -inducible NRT1.1-controlled GARP transcription factor, HRS1, document its genome-wide transcriptional targets, and validate its cis-regulatory elements. We demonstrate that this transcription factor and a close homologue repress the primary root growth in response to P deficiency conditions, but only when NO3- is present. This system defines a molecular logic gate integrating P and N signals. We propose that NO3- and P signalling converge via double transcriptional and post-transcriptional control of the same protein, HRS1.",

author = "Anna Medici and Amy Marshall-Colon and Elsa Ronzier and Wojciech Szponarski and Rongchen Wang and Alain Gojon and Crawford, {Nigel M.} and Sandrine Ruffel and Coruzzi, {Gloria M.} and Gabriel Krouk",

note = "Funding Information: We thank Beno{\^i}t Lacombe and Frederic Gaymard for the comments and discussions; Elodie Jublanc from the Montpellier Rio Imaging (MRI) core facility for the confocal assistance and three-dimensional imaging reconstruction; Tony Sierra for the technical assistance during Gene Atlas analysis; Veronique Santoni for the PIP2.1 antibody; and Franck Lecocq, Rogatien Picaud, Guy Ruiz, Thierry Dessup, and Hugues Baudot for the technical support and plant care. This work was supported by the French Agence Nationale de la Recherche (NitroNet: ANR 11 PDOC 020 01) and Centre National de la Recherche Scientifique (PEPS Bio math Info 2012–2013: SuperRegNet) to G.K. Work on the transcriptional networks was supported NIH R01-GM032877 to G.C. and NIH NRSA-GM095273 to A.M.-C. Results on root architecture responses are supported by NSF MCB-0929338 to G.C. Bioinformatics analysis was supported by the VirtualPlant platform (www.virtualplant.org) developed under NSF DBI-0445666 to G.C. Work by R.W. and N.C. was supported by the National Science Foundation grants (IOS-1021380 and MCB-0929338). Publisher Copyright: {\textcopyright} 2015 Macmillan Publishers Limited. All rights reserved.",

year = "2015",

month = feb,

day = "27",

doi = "10.1038/ncomms7274",

language = "English (US)",

volume = "6",

journal = "Nature Communications",

issn = "2041-1723",

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T1 - AtNIGT1/HRS1 integrates nitrate and phosphate signals at the Arabidopsis root tip

AU - Medici, Anna

AU - Marshall-Colon, Amy

AU - Ronzier, Elsa

AU - Szponarski, Wojciech

AU - Wang, Rongchen

AU - Gojon, Alain

AU - Crawford, Nigel M.

AU - Ruffel, Sandrine

AU - Coruzzi, Gloria M.

AU - Krouk, Gabriel

N1 - Funding Information: We thank Benoît Lacombe and Frederic Gaymard for the comments and discussions; Elodie Jublanc from the Montpellier Rio Imaging (MRI) core facility for the confocal assistance and three-dimensional imaging reconstruction; Tony Sierra for the technical assistance during Gene Atlas analysis; Veronique Santoni for the PIP2.1 antibody; and Franck Lecocq, Rogatien Picaud, Guy Ruiz, Thierry Dessup, and Hugues Baudot for the technical support and plant care. This work was supported by the French Agence Nationale de la Recherche (NitroNet: ANR 11 PDOC 020 01) and Centre National de la Recherche Scientifique (PEPS Bio math Info 2012–2013: SuperRegNet) to G.K. Work on the transcriptional networks was supported NIH R01-GM032877 to G.C. and NIH NRSA-GM095273 to A.M.-C. Results on root architecture responses are supported by NSF MCB-0929338 to G.C. Bioinformatics analysis was supported by the VirtualPlant platform (www.virtualplant.org) developed under NSF DBI-0445666 to G.C. Work by R.W. and N.C. was supported by the National Science Foundation grants (IOS-1021380 and MCB-0929338). Publisher Copyright: © 2015 Macmillan Publishers Limited. All rights reserved.

PY - 2015/2/27

Y1 - 2015/2/27

N2 - Nitrogen and phosphorus are among the most widely used fertilizers worldwide. Nitrate (NO3-) and phosphate (PO43-) are also signalling molecules whose respective transduction pathways are being intensively studied. However, plants are continuously challenged with combined nutritional deficiencies, yet very little is known about how these signalling pathways are integrated. Here we report the identification of a highly NO3- -inducible NRT1.1-controlled GARP transcription factor, HRS1, document its genome-wide transcriptional targets, and validate its cis-regulatory elements. We demonstrate that this transcription factor and a close homologue repress the primary root growth in response to P deficiency conditions, but only when NO3- is present. This system defines a molecular logic gate integrating P and N signals. We propose that NO3- and P signalling converge via double transcriptional and post-transcriptional control of the same protein, HRS1.

AB - Nitrogen and phosphorus are among the most widely used fertilizers worldwide. Nitrate (NO3-) and phosphate (PO43-) are also signalling molecules whose respective transduction pathways are being intensively studied. However, plants are continuously challenged with combined nutritional deficiencies, yet very little is known about how these signalling pathways are integrated. Here we report the identification of a highly NO3- -inducible NRT1.1-controlled GARP transcription factor, HRS1, document its genome-wide transcriptional targets, and validate its cis-regulatory elements. We demonstrate that this transcription factor and a close homologue repress the primary root growth in response to P deficiency conditions, but only when NO3- is present. This system defines a molecular logic gate integrating P and N signals. We propose that NO3- and P signalling converge via double transcriptional and post-transcriptional control of the same protein, HRS1.

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VL - 6

JO - Nature Communications

JF - Nature Communications

M1 - 6274

ER -

AtNIGT1/HRS1 integrates nitrate and phosphate signals at the Arabidopsis root tip

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