Atomic force microscope observation of branching in single transcript molecules derived from human cardiac muscle

Jason Reed, Carlin Hsueh, Bud Mishra, James K. Gimzewski

Research output: Contribution to journalArticle

Abstract

We have used an atomic force microscope to examine a clinically derived sample of single-molecule gene transcripts, in the form of double-stranded cDNA, (c: complementary) obtained from human cardiac muscle without the use of polymerase chain reaction (PCR) amplification. We observed a log-normal distribution of transcript sizes, with most molecules being in the range of 0.4-7.0 kilobase pairs (kb) or 130-2300 nm in contour length, in accordance with the expected distribution of mRNA (m: messenger) sizes in mammalian cells. We observed novel branching structures not previously known to exist in cDNA, and which could have profound negative effects on traditional analysis of cDNA samples through cloning, PCR and DNA sequencing.

Original languageEnglish (US)
Article number384021
JournalNanotechnology
Volume19
Issue number38
DOIs
StatePublished - Sep 24 2008

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Atomic force microscope observation of branching in single transcript molecules derived from human cardiac muscle'. Together they form a unique fingerprint.

  • Cite this