TY - JOUR
T1 - Atomic force microscopy of structures produced by electrospraying polymer solutions
AU - Morozov, Victor N.
AU - Morozova, Tamara Ya
AU - Kallenbach, Neville R.
N1 - Funding Information:
This work was supported by NSF grant no. BIR 9513571 and by NIH grant no. CA 24101.
PY - 1998/11/9
Y1 - 1998/11/9
N2 - Solutions of synthetic [poly(ethylene oxides), polyvinylpyrrolidone, polyacrylamide, and poly(vinylalcohol)] and natural linear polymers (single- and double-strand DNA) have been electrosprayed onto mica surface and imaged with an atomic force microscope. Depending on the electrospray ionization (ESI) conditions (concentration, solvent, sign of ion charge) each polymer studied could be imaged as globular or fibrillar forms. Globules were observed after electrospray (ES) of dilute solutions such as are routinely used in mass spectroscopy studies of polymers. An increase in the polymer concentration resulted in formation of larger multimolecular globular clusters, then extended fibers, presumably consisting of single (0.2-0.5 nm high) and multiple (>0.6 nm high) polymer strands. Further increase in the polymer concentration produced complex structures including branched fibers, "pins," and "beads-on-a-string." Formation of these structures is discussed in terms of known mechanisms of ESI.
AB - Solutions of synthetic [poly(ethylene oxides), polyvinylpyrrolidone, polyacrylamide, and poly(vinylalcohol)] and natural linear polymers (single- and double-strand DNA) have been electrosprayed onto mica surface and imaged with an atomic force microscope. Depending on the electrospray ionization (ESI) conditions (concentration, solvent, sign of ion charge) each polymer studied could be imaged as globular or fibrillar forms. Globules were observed after electrospray (ES) of dilute solutions such as are routinely used in mass spectroscopy studies of polymers. An increase in the polymer concentration resulted in formation of larger multimolecular globular clusters, then extended fibers, presumably consisting of single (0.2-0.5 nm high) and multiple (>0.6 nm high) polymer strands. Further increase in the polymer concentration produced complex structures including branched fibers, "pins," and "beads-on-a-string." Formation of these structures is discussed in terms of known mechanisms of ESI.
KW - Atomic force microscopy (AFM)
KW - Double stranded DNA
KW - Electrospray ionization
KW - Polymers
KW - Single stranded DNA
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U2 - 10.1016/S1387-3806(98)14083-6
DO - 10.1016/S1387-3806(98)14083-6
M3 - Article
AN - SCOPUS:0001638914
VL - 178
SP - 143
EP - 159
JO - International Journal of Mass Spectrometry and Ion Processes
JF - International Journal of Mass Spectrometry and Ion Processes
SN - 1387-3806
IS - 3
ER -