Automatic identification and delineation of germ layer components in H&E stained images of teratomas derived from human and nonhuman primate embryonic stem cells

Ramamurthy Bhagavatula, Matthew Fickus, W. Kelly, Chenlei Guo, John A. Ozolek, Carlos A. Castro, Jelena Kovačević

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a methodology for the automatic identification and delineation of germ-layer components in H&E stained images of teratomas derived from human and nonhuman primate embryonic stem cells. A knowledge and understanding of the biology of these cells may lead to advances in tissue regeneration and repair, the treatment of genetic and developmental syndromes, and drug testing and discovery. As a teratoma is a chaotic organization of tissues derived from the three primary embryonic germ layers, H&E teratoma images often present multiple tissues, each of having complex and unpredictable positions, shapes, and appearance with respect to each individual tissue as well as with respect to other tissues. While visual identification of these tissues is timeconsuming, it is surprisingly accurate, indicating that there exist enough visual cues to accomplish the task. We propose automatic identification and delineation of these tissues by mimicking these visual cues. We use pixel-based classification, resulting in an encouraging range of classification accuracies from 74.9% to 93.2% for 2- to 5-tissue classification experiments at different scales.

Original languageEnglish (US)
Title of host publication2010 7th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, ISBI 2010 - Proceedings
Pages1041-1044
Number of pages4
DOIs
StatePublished - 2010
Event7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Rotterdam, Netherlands
Duration: Apr 14 2010Apr 17 2010

Publication series

Name2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings

Other

Other7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010
CountryNetherlands
CityRotterdam
Period4/14/104/17/10

Keywords

  • Classification
  • Feature extraction
  • Image analysis
  • Stem cell biology

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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    Bhagavatula, R., Fickus, M., Kelly, W., Guo, C., Ozolek, J. A., Castro, C. A., & Kovačević, J. (2010). Automatic identification and delineation of germ layer components in H&E stained images of teratomas derived from human and nonhuman primate embryonic stem cells. In 2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings (pp. 1041-1044). [5490168] (2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings). https://doi.org/10.1109/ISBI.2010.5490168