@article{eb7137b9556445c99ca130b97123c9ab,
title = "Axon TRAP reveals learning-associated alterations in cortical axonal mRNAs in the lateral amgydala",
abstract = "Local translation can support memory consolidation by supplying new proteins to synapses undergoing plasticity. Translation in adult forebrain dendrites is an established mechanism of synaptic plasticity and is regulated by learning, yet there is no evidence for learning-regulated protein synthesis in adult forebrain axons, which have traditionally been believed to be incapable of translation. Here, we show that axons in the adult rat amygdala contain translation machinery, and use translating ribosome affinity purification (TRAP) with RNASeq to identify mRNAs in cortical axons projecting to the amygdala, over 1200 of which were regulated during consolidation of associative memory. Mitochondrial and translation-related genes were upregulated, whereas synaptic, cytoskeletal, and myelin-related genes were downregulated; the opposite effects were observed in the cortex. Our results demonstrate that axonal translation occurs in the adult forebrain and is altered after learning, supporting the likelihood that local translation is more a rule than an exception in neuronal processes.",
author = "Ostroff, {Linnaea E.} and Emanuela Santini and Robert Sears and Zachary Deane and Kanadia, {Rahul N.} and Ledoux, {Joseph E.} and Tenzin Lhakhang and Aristotelis Tsirigos and Adriana Heguy and Eric Klann",
note = "Funding Information: The L10a-YFP construct was a generous gift from Dr. Thomas Launey at the RIKEN Brain Science Institute, Tokyo, Japan. We are grateful to Yutong Zhang for expert technical assistance and to Drs. Joel Richter and Erin Schuman for their insightful comments on the manuscript. This work was supported by NIH grants MH119517, MH083583 and MH094965 to LO, NS087112 to ES, and NS034007, NS047384, and HD082013 to EK. We thank the Applied Bioinformatics Laboratories (ABL) at the NYU School of Medicine for providing bioinformatics support and helping with the analysis and interpretation of the data. This work has used computing resources at the NYU High-Perfor-mance Computing Facility (HPCF) and was supported in part by the Viral Vector Core of the Emory Neuroscience NINDS Core Facilities grant, P30NS055077. The Leica SP8 confocal used in this study was obtained with a grant from the NIH (S10OD016435) awarded to Akiko Nishiyama. National Institute of Neurological Disorders and Stroke NS034007 Eric Klann Eunice Kennedy Shriver National Institute of Child Health and Human Development HD082013. Eric Klann National Institute of Mental Health MH083583 Linnaea E Ostroff National Institute of Neurological Disorders and Stroke NS047384 Eric Klann National Institute of Mental Health MH094965 Linnaea E Ostroff National Institute of Mental Health MH119517 Linnaea E Ostroff National Institute of Neurological Disorders and Stroke NS087112 Emanuela Santini. Funding Information: The L10a-YFP construct was a generous gift from Dr. Thomas Launey at the RIKEN Brain Science Institute, Tokyo, Japan. We are grateful to Yutong Zhang for expert technical assistance and to Drs. Joel Richter and Erin Schuman for their insightful comments on the manuscript. This work was supported by NIH grants MH119517, MH083583 and MH094965 to LO, NS087112 to ES, and NS034007, NS047384, and HD082013 to EK. We thank the Applied Bioinformatics Laboratories (ABL) at the NYU School of Medicine for providing bioinformatics support and helping with the analysis and interpretation of the data. This work has used computing resources at the NYU High-Performance Computing Facility (HPCF) and was supported in part by the Viral Vector Core of the Emory Neuroscience NINDS Core Facilities grant, P30NS055077. The Leica SP8 confocal used in this study was obtained with a grant from the NIH (S10OD016435) awarded to Akiko Nishiyama. Publisher Copyright: {\textcopyright} 2019, eLife Sciences Publications Ltd. All rights reserved.",
year = "2019",
month = dec,
doi = "10.7554/eLife.51607",
language = "English (US)",
volume = "8",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}