@article{e9f050884f0e4236a60a45f20426555a,
title = "Axonal mRNA binding of hnRNP A/B is crucial for axon targeting and maturation of olfactory sensory neurons",
abstract = "Spatiotemporal control of gene expression is important for neural development and function. Here, we show that heterogeneous nuclear ribonucleoprotein (hnRNP) A/B is highly expressed in developing olfactory sensory neurons (OSNs), and its knockout results in reduction in mature OSNs and aberrant targeting of OSN axons to the olfactory bulb. RNA immunoprecipitation analysis reveals that hnRNP A/B binds to a group of mRNAs that are highly related to axon projections and synapse assembly. Approximately 11% of the identified hnRNP A/B targets, including Pcdha and Ncam2, encode cell adhesion molecules. In Hnrnpab knockout mice, PCDHA and NCAM2 levels are significantly reduced at the axon terminals of OSNs. Furthermore, deletion of the hnRNP A/B-recognition motif in the 3′ UTR of Pcdha leads to impaired PCDHA expression at the OSN axon terminals. Therefore, we propose that hnRNP A/B facilitates OSN maturation and axon projection by regulating the local expression of its target genes at axon terminals.",
keywords = "3′ UTR, CP: Developmental biology, CP: Neuroscience, RNA binding proteins, axon projections, axonal mRNA, cell adhesion molecules, hnRNPs, neural maturation, olfactory behavior, olfactory sensory neurons, post-transcriptional regulation",
author = "Nanaho Fukuda and Tomoyuki Fukuda and Piergiorgio Percipalle and Kanako Oda and Nobuyuki Takei and Kevin Czaplinski and Kazushige Touhara and Yoshihiro Yoshihara and Toshikuni Sasaoka",
note = "Funding Information: We are grateful to Dr. M. Kawaichi and Dr. Y. Ishida for valuable advice and material sharing and to A. Sasaki, M. Nozaki, and A. Hayashida for technical support. We thank Dr. D. Motooka (Genome Information Research Center, Osaka University) for next-generation sequencing (NGS) and analysis and Dr. E. Tarusawa and Dr. T. Yagi for sharing the antibody against PCDHA. We also thank all members of the animal facility at Niigata University and NAIST. This work was supported by JSPS KAKENHI grant numbers 22K06895 (N.F.), 19K07363 (N.F.), and 17K17884 (N.F.). N.F. received a grant from the Takeda Science Foundation . K.C. received National Science Foundation CAREER award ( IOS-1254146 ). Funding Information: We are grateful to Dr. M. Kawaichi and Dr. Y. Ishida for valuable advice and material sharing and to A. Sasaki, M. Nozaki, and A. Hayashida for technical support. We thank Dr. D. Motooka (Genome Information Research Center, Osaka University) for next-generation sequencing (NGS) and analysis and Dr. E. Tarusawa and Dr. T. Yagi for sharing the antibody against PCDHA. We also thank all members of the animal facility at Niigata University and NAIST. This work was supported by JSPS KAKENHI grant numbers 22K06895 (N.F.), 19K07363 (N.F.), and 17K17884 (N.F.). N.F. received a grant from the Takeda Science Foundation. K.C. received National Science Foundation CAREER award (IOS-1254146). Conceptualization, N.F. and T.F.; investigation, N.F.; methodology, K.O. and N.T.; writing – original draft, N.F.; writing – review & editing, T.F. Y.Y. and K.T.; funding acquisition, N.F.; resources, P.P. K.C. Y.Y. and T.S. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
month = may,
day = "30",
doi = "10.1016/j.celrep.2023.112398",
language = "English (US)",
volume = "42",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",
}