Benzo[a]pyrene-derived DNA lesions decrease DNA methylation by murine methyltransferase DNMT3A2

O. V. Lukashevich, N. A. Cherepanova, A. Kolbanovsky, N. E. Geacintov, E. S. Gromova

Research output: Contribution to journalArticle

Abstract

We have studied the impact of DNA damage by one of the most common carcinogens, benzo[a]pyrene, on the functioning of the truncated isoform of DNA methyltransferase Dnmt3a (Dnmt3a2). It is revealed with 30-mer model DNA substrates that DNA methylation rates are drastically reduced when the lesions disturb the structure of the Dnmt3a recognition site or hinder the interaction of the enzyme catalytic loop with the DNA minor groove. Under the chosen conditions, the PWWP domain of Dnmt3a possessing lower affinity to DNA in comparison with the catalytic domain does not influence catalysis.

Original languageEnglish (US)
Pages (from-to)92-96
Number of pages5
JournalMoscow University Chemistry Bulletin
Volume69
Issue number2
DOIs
StatePublished - Mar 2014

Keywords

  • DNA damage
  • DNA methyltransferase
  • benzo[a]pyrene
  • intercalation
  • methylation
  • minor groove

ASJC Scopus subject areas

  • Chemistry(all)

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