Abstract
We have studied the impact of DNA damage by one of the most common carcinogens, benzo[a]pyrene, on the functioning of the truncated isoform of DNA methyltransferase Dnmt3a (Dnmt3a2). It is revealed with 30-mer model DNA substrates that DNA methylation rates are drastically reduced when the lesions disturb the structure of the Dnmt3a recognition site or hinder the interaction of the enzyme catalytic loop with the DNA minor groove. Under the chosen conditions, the PWWP domain of Dnmt3a possessing lower affinity to DNA in comparison with the catalytic domain does not influence catalysis.
Original language | English (US) |
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Pages (from-to) | 92-96 |
Number of pages | 5 |
Journal | Moscow University Chemistry Bulletin |
Volume | 69 |
Issue number | 2 |
DOIs | |
State | Published - Mar 2014 |
Keywords
- DNA damage
- DNA methyltransferase
- benzo[a]pyrene
- intercalation
- methylation
- minor groove
ASJC Scopus subject areas
- General Chemistry