Flow-based microfluidic biochips (FMBs) have microvalves as key components. The physical characteristics of the microvalves vary instance-to-instance due to the inherent variability of numerous fabrication parameters. In this work, we leverage this unclonable, unpredictable instance-specific behavior and propose physically unclonable functions (PUFs) for FMBs, namely Biochip-PUFs (Bio-PUFs in short). We utilize variability in the microvalve membrane deflection response associated with the actuation pressure challenge to be our Bio-PUF parameter. Based on the distributions of the parameters measured on actual FMBs, we complement our Bio-PUF measurements via simulations of the FMB's microvalves in Comsol Multiphysics. Furthermore, we present a scheme based on the transient response of the microvalve actuation to augment the Bio-PUF authentication. The major advantage of this scheme is that we do not need any additional hardware to generate/implement the PUF module. The biochip itself can act as PUF instances while continuing to operate in normal functioning mode.