Biochip-PUF: Physically Unclonable Function for Microfluidic Biochips

Navajit Singh Baban, Ajymurat Orozaliev, Yong Ak Song, Urbi Chatterjee, Sankalp Bose, Sukanta Bhattacharjee, Ramesh Karri, Krishnendu Chakrabarty

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Flow-based microfluidic biochips (FMBs) have microvalves as key components. The physical characteristics of the microvalves vary instance-to-instance due to the inherent variability of numerous fabrication parameters. In this work, we leverage this unclonable, unpredictable instance-specific behavior and propose physically unclonable functions (PUFs) for FMBs, namely Biochip-PUFs (Bio-PUFs in short). We utilize variability in the microvalve membrane deflection response associated with the actuation pressure challenge to be our Bio-PUF parameter. Based on the distributions of the parameters measured on actual FMBs, we complement our Bio-PUF measurements via simulations of the FMB's microvalves in Comsol Multiphysics. Furthermore, we present a scheme based on the transient response of the microvalve actuation to augment the Bio-PUF authentication. The major advantage of this scheme is that we do not need any additional hardware to generate/implement the PUF module. The biochip itself can act as PUF instances while continuing to operate in normal functioning mode.

Original languageEnglish (US)
Title of host publicationProceedings - 2023 IEEE International Test Conference, ITC 2023
PublisherInstitute of Electrical and Electronics Engineers Inc.
Number of pages10
ISBN (Electronic)9798350343250
StatePublished - 2023
Event2023 IEEE International Test Conference, ITC 2023 - Anaheim, United States
Duration: Oct 7 2023Oct 15 2023

Publication series

NameProceedings - International Test Conference
ISSN (Print)1089-3539


Conference2023 IEEE International Test Conference, ITC 2023
Country/TerritoryUnited States


  • Authentication
  • Biochips
  • Cyber-physical System
  • Intellectual Property
  • Microfluidics
  • Microvalves
  • Physically Unclonable Functions

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Applied Mathematics


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