Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells

Irene R. Dégano; Marta Vilalta; Juli R. Bagó; Annette M. Matthies; Jeffrey A. Hubbell; Helen Dimitriou; Paolo Bianco; Nuria Rubio; Jerónimo Blanco

doi:10.1016/j.biomaterials.2007.10.006

Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells

Irene R. Dégano, Marta Vilalta, Juli R. Bagó, Annette M. Matthies, Jeffrey A. Hubbell, Helen Dimitriou, Paolo Bianco, Nuria Rubio, Jerónimo Blanco

Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

A combined strategy using bioluminescence imaging, bone densitometry and histology was used to analyze the bone regeneration capacity of human bone marrow (hBMSC) and adipose tissue (hAMSC) mesenchymal stem cells, seeded in an osteoconductive arginine-glycine-aspartate (RGD) crosslinked hydrogel scaffold, implanted in a mouse calvarial bone defect. We show that firefly luciferase labeled stem cells can be monitored in vivo through a prolonged 90 days period, during which hBMSCs survive better than hAMSCs and that the density of scaffold bearing defects increased significantly more than that of defects without scaffolds.

Original language	English (US)
Pages (from-to)	427-437
Number of pages	11
Journal	Biomaterials
Volume	29
Issue number	4
DOIs	https://doi.org/10.1016/j.biomaterials.2007.10.006
State	Published - Feb 2008

Keywords

Cell differentiation
Enhanced green fluorescent protein (eGFP)
In vivo imaging
Luciferase
Scaffold

ASJC Scopus subject areas

Biophysics
Bioengineering
Ceramics and Composites
Biomaterials
Mechanics of Materials

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10.1016/j.biomaterials.2007.10.006

Cite this

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title = "Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells",

abstract = "A combined strategy using bioluminescence imaging, bone densitometry and histology was used to analyze the bone regeneration capacity of human bone marrow (hBMSC) and adipose tissue (hAMSC) mesenchymal stem cells, seeded in an osteoconductive arginine-glycine-aspartate (RGD) crosslinked hydrogel scaffold, implanted in a mouse calvarial bone defect. We show that firefly luciferase labeled stem cells can be monitored in vivo through a prolonged 90 days period, during which hBMSCs survive better than hAMSCs and that the density of scaffold bearing defects increased significantly more than that of defects without scaffolds.",

keywords = "Cell differentiation, Enhanced green fluorescent protein (eGFP), In vivo imaging, Luciferase, Scaffold",

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doi = "10.1016/j.biomaterials.2007.10.006",

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T1 - Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells

AU - Dégano, Irene R.

AU - Vilalta, Marta

AU - Bagó, Juli R.

AU - Matthies, Annette M.

AU - Hubbell, Jeffrey A.

AU - Dimitriou, Helen

AU - Bianco, Paolo

AU - Rubio, Nuria

AU - Blanco, Jerónimo

PY - 2008/2

Y1 - 2008/2

N2 - A combined strategy using bioluminescence imaging, bone densitometry and histology was used to analyze the bone regeneration capacity of human bone marrow (hBMSC) and adipose tissue (hAMSC) mesenchymal stem cells, seeded in an osteoconductive arginine-glycine-aspartate (RGD) crosslinked hydrogel scaffold, implanted in a mouse calvarial bone defect. We show that firefly luciferase labeled stem cells can be monitored in vivo through a prolonged 90 days period, during which hBMSCs survive better than hAMSCs and that the density of scaffold bearing defects increased significantly more than that of defects without scaffolds.

AB - A combined strategy using bioluminescence imaging, bone densitometry and histology was used to analyze the bone regeneration capacity of human bone marrow (hBMSC) and adipose tissue (hAMSC) mesenchymal stem cells, seeded in an osteoconductive arginine-glycine-aspartate (RGD) crosslinked hydrogel scaffold, implanted in a mouse calvarial bone defect. We show that firefly luciferase labeled stem cells can be monitored in vivo through a prolonged 90 days period, during which hBMSCs survive better than hAMSCs and that the density of scaffold bearing defects increased significantly more than that of defects without scaffolds.

KW - Cell differentiation

KW - Enhanced green fluorescent protein (eGFP)

KW - In vivo imaging

KW - Luciferase

KW - Scaffold

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JO - Biomaterials

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Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells

Abstract

Keywords

ASJC Scopus subject areas

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