Biomedical diagnostic with diffusely backscattered linearly and circularly polarized light

Andreas H. Hielscher, Angelia A. Eick, Judith R. Mourant, James P. Freyer, Irving J. Bigio

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report on the development of a method that records spatially dependent intensity patterns of polarized light that is diffusely backscattered from turbid materials. It is demonstrated that these intensity patterns can be used to differentiate between suspensions of cancerous and non-cancerous cells and to observe different metabolic cell processes. Our technique employs polarized light from a He-Ne laser (λ= 543 nm), which is focused onto the surface of the scattering medium. A surface area of approximately 4×4 cm around the light input point is imaged through polarization-analysis optics onto a CCD camera. One can observe a manifold of intensity patterns by varying the polarization state of the incident laser light and changing the analyzer configuration to detect different polarization components of the backscattered light. Introducing the Mueller-matrix concept for diffusely backscattered light, a framework is provided to select a subset of measurements which comprehensively describe the optical properties of backscattering media.

Original languageEnglish (US)
Title of host publicationProceedings of Biomedical Sensing, Imaging and Tracking Technologies II
Subtitle of host publicationProceedings of SPIE - The International Society for Optical Engineering
Pages298-305
Number of pages8
Volume2976
DOIs
StatePublished - 1997
EventProceedings of Biomedical Sensing, Imaging and Tracking Technologies II - San Jose, CA, United States
Duration: Feb 11 1997Feb 13 1997

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
PublisherSPIE
ISSN (Print)0277-786X

Conference

ConferenceProceedings of Biomedical Sensing, Imaging and Tracking Technologies II
Country/TerritoryUnited States
CitySan Jose, CA
Period2/11/972/13/97

Keywords

  • Backscattering
  • Biological-cell suspensions
  • Cancer
  • Fibroblasts
  • Mueller matrix
  • Polarization
  • Polystyrene spheres
  • Stokes vector

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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