TY - GEN
T1 - Biophysical Analysis of Drug Efficacy on C. elegans Models for Neurodegenerative and Neuromuscular Diseases
AU - Sofela, Samuel
AU - Sahloul, Sarah
AU - Song, Yong Ak
N1 - Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - We report the use of a micropillar-based force assay chip for C. elegans in combination with a fluorescence assay to evaluate the efficacy of various drugs for treatment of neurodegenerative and neuromuscular diseases. Using an array of flexible micropillars and subsequent FEM analysis, the thrashing force of C. elegans models for Duchenne Muscular Dystrophy (DMD), Amyotrophic Lateral Sclerosis (ALS), and Parkinson's disease (PD) has been measured before and after treatment with drugs and their efficacy quantified. The force assay chip allowed quantifying force changes upon drug treatment accurately and could be used in high-throughput drug screening assays for muscle-related diseases.
AB - We report the use of a micropillar-based force assay chip for C. elegans in combination with a fluorescence assay to evaluate the efficacy of various drugs for treatment of neurodegenerative and neuromuscular diseases. Using an array of flexible micropillars and subsequent FEM analysis, the thrashing force of C. elegans models for Duchenne Muscular Dystrophy (DMD), Amyotrophic Lateral Sclerosis (ALS), and Parkinson's disease (PD) has been measured before and after treatment with drugs and their efficacy quantified. The force assay chip allowed quantifying force changes upon drug treatment accurately and could be used in high-throughput drug screening assays for muscle-related diseases.
KW - C. elegans
KW - biophysical phenotyping
KW - drug screening
KW - neurodegenerative diseases
KW - neuromuscular diseases
UR - http://www.scopus.com/inward/record.url?scp=85136955942&partnerID=8YFLogxK
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M3 - Conference contribution
AN - SCOPUS:85136955942
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 391
EP - 392
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -