TY - JOUR
T1 - Bone apposition to laminin-1 coated implants
T2 - Histologic and 3D evaluation
AU - Bougas, K.
AU - Jimbo, R.
AU - Vandeweghe, S.
AU - Hayashi, M.
AU - Bryington, M.
AU - Kozai, Y.
AU - Schwartz-Filho, H. O.
AU - Tovar, N.
AU - Adolfsson, E.
AU - Ono, D.
AU - Coelho, P. G.
AU - Wennerberg, A.
N1 - Funding Information:
This study was supported by the Swedish Knowledge Foundation , the Swedish Research Council , the Hjalmar Svensson Research Foundation , and the Swedish National Graduate School in Odontological Science .
PY - 2013/5
Y1 - 2013/5
N2 - Laminin-1 has been reported as one of the factors responsible for the nucleation of calcium phosphates and, in vitro, has been reported to selectively recruit osteoprogenitors. This article focused on its in vivo effects, and evaluated the effect of laminin-1 local application on osseointegration. Polished cylindrical hydroxyapatite implants were coated with laminin-1 (test) and the bone responses in the rabbit tibiae after 2 and 4 weeks were evaluated and compared to the non-coated implants (control). Before the samples were processed for histological sectioning, they were three-dimensionally analysed with micro computed tomography (μCT). Both evaluation methods were analysed with regards to bone area around the implant and bone to implant contact. From the histologic observation, new bone formation around the laminin-1 coated implant at 2 weeks seemed to have increased the amount of supporting bone around the implant, however, at 4 weeks, the two groups presented no notable differences. The two-dimensional and three-dimensional morphometric evaluation revealed that both histologic and three-dimensional analysis showed some tendency in favour of the test group implants, however there was no statistical significance between the test and control group results.
AB - Laminin-1 has been reported as one of the factors responsible for the nucleation of calcium phosphates and, in vitro, has been reported to selectively recruit osteoprogenitors. This article focused on its in vivo effects, and evaluated the effect of laminin-1 local application on osseointegration. Polished cylindrical hydroxyapatite implants were coated with laminin-1 (test) and the bone responses in the rabbit tibiae after 2 and 4 weeks were evaluated and compared to the non-coated implants (control). Before the samples were processed for histological sectioning, they were three-dimensionally analysed with micro computed tomography (μCT). Both evaluation methods were analysed with regards to bone area around the implant and bone to implant contact. From the histologic observation, new bone formation around the laminin-1 coated implant at 2 weeks seemed to have increased the amount of supporting bone around the implant, however, at 4 weeks, the two groups presented no notable differences. The two-dimensional and three-dimensional morphometric evaluation revealed that both histologic and three-dimensional analysis showed some tendency in favour of the test group implants, however there was no statistical significance between the test and control group results.
KW - animal experiments
KW - bone implant interactions
KW - laminin
KW - micro CT
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U2 - 10.1016/j.ijom.2012.11.008
DO - 10.1016/j.ijom.2012.11.008
M3 - Article
C2 - 23228694
AN - SCOPUS:84876115468
SN - 0901-5027
VL - 42
SP - 677
EP - 682
JO - International Journal of Oral and Maxillofacial Surgery
JF - International Journal of Oral and Maxillofacial Surgery
IS - 5
ER -