Breaking the degeneracy of the genetic code

Inchan Kwon, Kent Kirshenbaum, David A. Tirrell

Research output: Contribution to journalArticlepeer-review


A mutant yeast phenylalanine transfer RNA (ytRNAPheAAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNAPheAAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.

Original languageEnglish (US)
Pages (from-to)7512-7513
Number of pages2
JournalJournal of the American Chemical Society
Issue number25
StatePublished - Jun 25 2003

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry


Dive into the research topics of 'Breaking the degeneracy of the genetic code'. Together they form a unique fingerprint.

Cite this