Breaking the degeneracy of the genetic code

Inchan Kwon; Kent Kirshenbaum; David A. Tirrell

doi:10.1021/ja0350076

Breaking the degeneracy of the genetic code

Inchan Kwon, Kent Kirshenbaum, David A. Tirrell

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

A mutant yeast phenylalanine transfer RNA (ytRNA^Phe_AAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNA^Phe_AAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.

Original language	English (US)
Pages (from-to)	7512-7513
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	125
Issue number	25
DOIs	https://doi.org/10.1021/ja0350076
State	Published - Jun 25 2003

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja0350076

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Breaking the degeneracy of the genetic code

Abstract

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