Breaking the degeneracy of the genetic code

Inchan Kwon, Kent Kirshenbaum, David A. Tirrell

Research output: Contribution to journalArticlepeer-review

Abstract

A mutant yeast phenylalanine transfer RNA (ytRNAPheAAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNAPheAAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.

Original languageEnglish (US)
Pages (from-to)7512-7513
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number25
DOIs
StatePublished - Jun 25 2003

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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