Calcium modulation of mitochondrial inner membrane channel activity

Kathleen W. Kinnally; Zorov Dmitry; Yuri Antonenko; Sean Perini

doi:10.1016/0006-291X(91)90410-9

Calcium modulation of mitochondrial inner membrane channel activity

Kathleen W. Kinnally, Zorov Dmitry, Yuri Antonenko, Sean Perini

Research output: Contribution to journal › Article › peer-review

Abstract

Protocols were defined that enable patch-clamp studies of the ∼107 pS voltage dependent channel and a class of activity we refer to as MCC (multiconductance channel) which is characterized by multiple levels and transitions as high as 1 to 1.5 nS. If free calcium was kept at 10^-7 M or lower during mitochondrial isolation, no activity was observed at low voltage (±60 mV). If free calcium levels were higher, MCC activity was observed in about 96% of the patches. The observation of ∼ 107 pS channel was enhanced from 2% to 68% of patches by washing isolated mitoplasts (mitochondria stripped of outer membrane) with EGTA. Increasing matrix calcium from 10^-9 to 10^-5 M decreased the probability of opening for the MCC and ∼107 pS activities.

Original language	English (US)
Pages (from-to)	1183-1188
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	176
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(91)90410-9
State	Published - May 15 1991

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(91)90410-9

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@article{98586f60ffa344f1b788cfd93114ca25,

title = "Calcium modulation of mitochondrial inner membrane channel activity",

abstract = "Protocols were defined that enable patch-clamp studies of the ∼107 pS voltage dependent channel and a class of activity we refer to as MCC (multiconductance channel) which is characterized by multiple levels and transitions as high as 1 to 1.5 nS. If free calcium was kept at 10-7 M or lower during mitochondrial isolation, no activity was observed at low voltage (±60 mV). If free calcium levels were higher, MCC activity was observed in about 96% of the patches. The observation of ∼ 107 pS channel was enhanced from 2% to 68% of patches by washing isolated mitoplasts (mitochondria stripped of outer membrane) with EGTA. Increasing matrix calcium from 10-9 to 10-5 M decreased the probability of opening for the MCC and ∼107 pS activities.",

author = "Kinnally, {Kathleen W.} and Zorov Dmitry and Yuri Antonenko and Sean Perini",

note = "Funding Information: We thank Henry Tedeschi for his support and helpful advice and Douglas Pfeiffer and Kimberly Broekemeier for their frequent discussions. This work was supported in pati by NSF grant DCB-8818432, USIA IA-AEMP-G8193395 and USIA IA-AEMP-G 193692.",

year = "1991",

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doi = "10.1016/0006-291X(91)90410-9",

language = "English (US)",

volume = "176",

pages = "1183--1188",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Calcium modulation of mitochondrial inner membrane channel activity

AU - Kinnally, Kathleen W.

AU - Dmitry, Zorov

AU - Antonenko, Yuri

AU - Perini, Sean

N1 - Funding Information: We thank Henry Tedeschi for his support and helpful advice and Douglas Pfeiffer and Kimberly Broekemeier for their frequent discussions. This work was supported in pati by NSF grant DCB-8818432, USIA IA-AEMP-G8193395 and USIA IA-AEMP-G 193692.

PY - 1991/5/15

Y1 - 1991/5/15

N2 - Protocols were defined that enable patch-clamp studies of the ∼107 pS voltage dependent channel and a class of activity we refer to as MCC (multiconductance channel) which is characterized by multiple levels and transitions as high as 1 to 1.5 nS. If free calcium was kept at 10-7 M or lower during mitochondrial isolation, no activity was observed at low voltage (±60 mV). If free calcium levels were higher, MCC activity was observed in about 96% of the patches. The observation of ∼ 107 pS channel was enhanced from 2% to 68% of patches by washing isolated mitoplasts (mitochondria stripped of outer membrane) with EGTA. Increasing matrix calcium from 10-9 to 10-5 M decreased the probability of opening for the MCC and ∼107 pS activities.

AB - Protocols were defined that enable patch-clamp studies of the ∼107 pS voltage dependent channel and a class of activity we refer to as MCC (multiconductance channel) which is characterized by multiple levels and transitions as high as 1 to 1.5 nS. If free calcium was kept at 10-7 M or lower during mitochondrial isolation, no activity was observed at low voltage (±60 mV). If free calcium levels were higher, MCC activity was observed in about 96% of the patches. The observation of ∼ 107 pS channel was enhanced from 2% to 68% of patches by washing isolated mitoplasts (mitochondria stripped of outer membrane) with EGTA. Increasing matrix calcium from 10-9 to 10-5 M decreased the probability of opening for the MCC and ∼107 pS activities.

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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ER -

Calcium modulation of mitochondrial inner membrane channel activity

Abstract

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