@article{824c686dade54c2e8ad9390d3d089b88,
title = "Camphor white oil induces tumor regression through cytotoxic T cell-dependent mechanisms",
abstract = "Bioactive derivatives from the camphor laurel tree, Cinnamomum camphora, are posited to exhibit chemopreventive properties but the efficacy and mechanism of these natural products are not fully understood. We tested an essential-oil derivative, camphor white oil (CWO), for anti-tumor activity in a mouse model of keratinocyte-derived skin cancer. Daily topical treatment with CWO induced dramatic regression of pre-malignant skin tumors and a two-fold reduction in cutaneous squamous cell carcinomas. We next investigated underlying cellular and molecular mechanisms. In cultured keratinocytes, CWO stimulated calcium signaling, resulting in calcineurin-dependent activation of nuclear factor of activated T cells (NFAT). In vivo, CWO induced transcriptional changes in immune-related genes identified by RNA-sequencing, resulting in cytotoxic T cell-dependent tumor regression. Finally, we identified chemical constituents of CWO that recapitulated effects of the admixture. Together, these studies identify T cell-mediated tumor regression as a mechanism through which a plant-derived essential oil diminishes established tumor burden.",
keywords = "calcium, camphor white oil, inflammation, NFAT, squamous cell carcinoma",
author = "Yalda Moayedi and Greenberg, {Sophie A.} and Jenkins, {Blair A.} and Marshall, {Kara L.} and Dimitrov, {Lina V.} and Nelson, {Aislyn M.} and Owens, {David M.} and Lumpkin, {Ellen A.}",
note = "Funding Information: We thank Yan Lu and Milda Stanislauskas for assistance with histology, Rong Du for assistance with cell culture, Spandan Shah and Siu-Hong Ho for flow cytometry, Dr. Diana Bautista and Carolyn Walsh for helpful discussions, Dr. Masashi Nakatani for preliminary studies of TRPV3 knockout mice, and Dr. Lan Li for critical reading of the manuscript. Funding was provided by NIH/NIAMS R01AR051219 (to EAL) and a pilot award from NIH/NIEHS (P30ES009089). Cell culture and microscopy were performed with support from the Columbia University Skin Disease Resource-Based Center (epiCURE, P30AR069632). RNA sequencing was performed with support from the JP Sulzberger Columbia Genome Center Core (P30CA013696). Flow cytometry was performed using the Columbia Center for Translational Immunology (CCTI) Core facility (P30CA013696). SAG was funded by NIH/NIAMS P30AR044535, Columbia University Dean's Research Fellowship, and NIH/NHLBI 5T35HL007616. YM was funded by NIH/NHBLI 1T32HL120826. Funding Information: We thank Yan Lu and Milda Stanislauskas for assistance with histology, Rong Du for assistance with cell culture, Spandan Shah and Siu-Hong Ho for flow cytometry, Dr. Diana Bautista and Carolyn Walsh for helpful discussions, Dr. Masashi Nakatani for preliminary studies of TRPV3 knockout mice, and Dr. Lan Li for critical reading of the manuscript. Funding was provided by NIH/ NIAMS R01AR051219 (to EAL) and a pilot award from NIH/NIEHS (P30ES009089). Cell culture and microscopy were performed with support from the Columbia University Skin Disease Resource-Based Center (epiCURE, P30AR069632). RNA sequencing was performed with support from the JP Sulzberger Columbia Genome Center Core (P30CA013696). Flow cytometry was performed using the Columbia Center for Translational Immunology (CCTI) Core facility (P30CA013696). SAG was funded by NIH/NIAMS P30AR044535, Columbia University Dean's Research Fellowship, and NIH/NHLBI 5T35HL007616. YM was funded by NIH/NHBLI 1T32HL120826. Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",
year = "2019",
month = may,
doi = "10.1002/mc.22965",
language = "English (US)",
volume = "58",
pages = "722--734",
journal = "Molecular Carcinogenesis",
issn = "0899-1987",
publisher = "Wiley-Liss Inc.",
number = "5",
}