Ca2+ binding properties of type X collagen

Thorsten Kirsch, Klaus von der Mark

Research output: Contribution to journalArticlepeer-review


Type X collagen is a developmentally regulated collagen that is only synthesized by chondrocytes of the hypertrophic and calcifying zone in fetal cartilage. There is evidence in the literature that type X collagen may be involved in cartilage calcification. Here we show that type X collagen synthesis precedes calcium deposition in nodules of fetal human chondrocytes forming in cell culture and present evidence that type X collagen binds calcium in a specific and dose dependent manner. In an assay using bovine type X collagen coupled to beads and 45Ca2+ we determined a total of about 15 binding sites per α1(X) chain with a dissociation constant of 32 μM.

Original languageEnglish (US)
Pages (from-to)149-152
Number of pages4
JournalFEBS Letters
Issue number1-2
StatePublished - Dec 2 1991


  • Calcium binding
  • Cartilage calcification
  • Chondrocyte hypertrophy
  • Type X collagen

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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