The aim of this study was to develop a method to examine the catabolism of neuropeptides in the tissue fluids that bathe specific regions in the alimentary tract of the conscious animal. Two bundles of dialysis fibers were surgically implanted in the submucosal region of the gastric corpus in the anesthetized rat. After recovery from surgery, one set of fibers was perfused with 125I - Tyr4 bombesin 14. Some molecules of peptide diffused into the tissues and were catabolized by enzymes therein. The catabolites were collected in the second dialysis fiber and fractionated by high pressure liquid chromatography. Bombesin 14 was catabolized to iodo-tyrosine and three unidentified peptides. The catabolites did not bind with a carboxyl-terminal specific antiserum and, as this is the biologically active region of bombesin, were devoid of any biological activity.
ASJC Scopus subject areas
- Endocrine and Autonomic Systems
- Cellular and Molecular Neuroscience