Catabolism of bombesin in the interstitial fluid of the rat stomach

Nigel W. Bunnett; Joseph R. Reeve; John H. Walsh

doi:10.1016/0143-4179(83)90009-4

Catabolism of bombesin in the interstitial fluid of the rat stomach

Nigel W. Bunnett, Joseph R. Reeve, John H. Walsh

Molecular Pathobiology

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to develop a method to examine the catabolism of neuropeptides in the tissue fluids that bathe specific regions in the alimentary tract of the conscious animal. Two bundles of dialysis fibers were surgically implanted in the submucosal region of the gastric corpus in the anesthetized rat. After recovery from surgery, one set of fibers was perfused with ¹²⁵I - Tyr4 bombesin 14. Some molecules of peptide diffused into the tissues and were catabolized by enzymes therein. The catabolites were collected in the second dialysis fiber and fractionated by high pressure liquid chromatography. Bombesin 14 was catabolized to iodo-tyrosine and three unidentified peptides. The catabolites did not bind with a carboxyl-terminal specific antiserum and, as this is the biologically active region of bombesin, were devoid of any biological activity.

Original language	English (US)
Pages (from-to)	55-64
Number of pages	10
Journal	Neuropeptides
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/0143-4179(83)90009-4
State	Published - Dec 1983

ASJC Scopus subject areas

Endocrinology
Neurology
Endocrine and Autonomic Systems
Cellular and Molecular Neuroscience

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10.1016/0143-4179(83)90009-4

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title = "Catabolism of bombesin in the interstitial fluid of the rat stomach",

abstract = "The aim of this study was to develop a method to examine the catabolism of neuropeptides in the tissue fluids that bathe specific regions in the alimentary tract of the conscious animal. Two bundles of dialysis fibers were surgically implanted in the submucosal region of the gastric corpus in the anesthetized rat. After recovery from surgery, one set of fibers was perfused with 125I - Tyr4 bombesin 14. Some molecules of peptide diffused into the tissues and were catabolized by enzymes therein. The catabolites were collected in the second dialysis fiber and fractionated by high pressure liquid chromatography. Bombesin 14 was catabolized to iodo-tyrosine and three unidentified peptides. The catabolites did not bind with a carboxyl-terminal specific antiserum and, as this is the biologically active region of bombesin, were devoid of any biological activity.",

author = "Bunnett, {Nigel W.} and Reeve, {Joseph R.} and Walsh, {John H.}",

note = "Funding Information: Arlene Schneider and Sharon Heil typed the manuscript and illustrations are by Helen Corbet. Supported by NIH grant number AM 17294 and by VA research funds.",

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AU - Bunnett, Nigel W.

AU - Reeve, Joseph R.

AU - Walsh, John H.

N1 - Funding Information: Arlene Schneider and Sharon Heil typed the manuscript and illustrations are by Helen Corbet. Supported by NIH grant number AM 17294 and by VA research funds.

PY - 1983/12

Y1 - 1983/12

N2 - The aim of this study was to develop a method to examine the catabolism of neuropeptides in the tissue fluids that bathe specific regions in the alimentary tract of the conscious animal. Two bundles of dialysis fibers were surgically implanted in the submucosal region of the gastric corpus in the anesthetized rat. After recovery from surgery, one set of fibers was perfused with 125I - Tyr4 bombesin 14. Some molecules of peptide diffused into the tissues and were catabolized by enzymes therein. The catabolites were collected in the second dialysis fiber and fractionated by high pressure liquid chromatography. Bombesin 14 was catabolized to iodo-tyrosine and three unidentified peptides. The catabolites did not bind with a carboxyl-terminal specific antiserum and, as this is the biologically active region of bombesin, were devoid of any biological activity.

AB - The aim of this study was to develop a method to examine the catabolism of neuropeptides in the tissue fluids that bathe specific regions in the alimentary tract of the conscious animal. Two bundles of dialysis fibers were surgically implanted in the submucosal region of the gastric corpus in the anesthetized rat. After recovery from surgery, one set of fibers was perfused with 125I - Tyr4 bombesin 14. Some molecules of peptide diffused into the tissues and were catabolized by enzymes therein. The catabolites were collected in the second dialysis fiber and fractionated by high pressure liquid chromatography. Bombesin 14 was catabolized to iodo-tyrosine and three unidentified peptides. The catabolites did not bind with a carboxyl-terminal specific antiserum and, as this is the biologically active region of bombesin, were devoid of any biological activity.

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Catabolism of bombesin in the interstitial fluid of the rat stomach

Abstract

ASJC Scopus subject areas

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