Catabolism of calcitonin gene-related peptide and substance P by neutral endopeptidase

M. Katayama; J. A. Nadel; N. W. Bunnett; G. U. Di Maria; M. Haxhiu; D. B. Borson

doi:10.1016/0196-9781(91)90102-U

Catabolism of calcitonin gene-related peptide and substance P by neutral endopeptidase

M. Katayama, J. A. Nadel, N. W. Bunnett, G. U. Di Maria, M. Haxhiu, D. B. Borson

Molecular Pathobiology

Research output: Contribution to journal › Article › peer-review

Abstract

Calcitonin gene-related peptide (CGRP) and substance P (SP) are released from sensory nerves upon exposure to irritating stimuli. Neutral endopeptidase (NEP), a membrane-bound peptidase, cleaves many peptides including SP, thereby limiting their biological actions. Recombinant NEP cleaved CGRP₁ approximately 88-fold less rapidly than it cleaved SP. The slow cleavage by NEP of CGRP compared to SP suggests that this enzyme is likely to have weaker physiologic effects on CGRP than have been demonstrated for SP.

Original language	English (US)
Pages (from-to)	563-567
Number of pages	5
Journal	Peptides
Volume	12
Issue number	3
DOIs	https://doi.org/10.1016/0196-9781(91)90102-U
State	Published - 1991

Keywords

CALLA
CD10
E.C.3.4.24.11.
Enkephalinase
Enzyme kinetics
HPLC
Neutral metalloendopeptidase
Peptidase inhibitors
Peptidases

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/0196-9781(91)90102-U

Cite this

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title = "Catabolism of calcitonin gene-related peptide and substance P by neutral endopeptidase",

abstract = "Calcitonin gene-related peptide (CGRP) and substance P (SP) are released from sensory nerves upon exposure to irritating stimuli. Neutral endopeptidase (NEP), a membrane-bound peptidase, cleaves many peptides including SP, thereby limiting their biological actions. Recombinant NEP cleaved CGRP1 approximately 88-fold less rapidly than it cleaved SP. The slow cleavage by NEP of CGRP compared to SP suggests that this enzyme is likely to have weaker physiologic effects on CGRP than have been demonstrated for SP.",

keywords = "CALLA, CD10, E.C.3.4.24.11., Enkephalinase, Enzyme kinetics, HPLC, Neutral metalloendopeptidase, Peptidase inhibitors, Peptidases",

author = "M. Katayama and Nadel, {J. A.} and Bunnett, {N. W.} and {Di Maria}, {G. U.} and M. Haxhiu and Borson, {D. B.}",

note = "Funding Information: The authors thank B. Raymond and I. F. Ueki for technical assistance. Study supported in part by HL-38947, HL-24136, and DK-39957.",

year = "1991",

doi = "10.1016/0196-9781(91)90102-U",

language = "English (US)",

volume = "12",

pages = "563--567",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "3",

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TY - JOUR

T1 - Catabolism of calcitonin gene-related peptide and substance P by neutral endopeptidase

AU - Katayama, M.

AU - Nadel, J. A.

AU - Bunnett, N. W.

AU - Di Maria, G. U.

AU - Haxhiu, M.

AU - Borson, D. B.

N1 - Funding Information: The authors thank B. Raymond and I. F. Ueki for technical assistance. Study supported in part by HL-38947, HL-24136, and DK-39957.

PY - 1991

Y1 - 1991

N2 - Calcitonin gene-related peptide (CGRP) and substance P (SP) are released from sensory nerves upon exposure to irritating stimuli. Neutral endopeptidase (NEP), a membrane-bound peptidase, cleaves many peptides including SP, thereby limiting their biological actions. Recombinant NEP cleaved CGRP1 approximately 88-fold less rapidly than it cleaved SP. The slow cleavage by NEP of CGRP compared to SP suggests that this enzyme is likely to have weaker physiologic effects on CGRP than have been demonstrated for SP.

AB - Calcitonin gene-related peptide (CGRP) and substance P (SP) are released from sensory nerves upon exposure to irritating stimuli. Neutral endopeptidase (NEP), a membrane-bound peptidase, cleaves many peptides including SP, thereby limiting their biological actions. Recombinant NEP cleaved CGRP1 approximately 88-fold less rapidly than it cleaved SP. The slow cleavage by NEP of CGRP compared to SP suggests that this enzyme is likely to have weaker physiologic effects on CGRP than have been demonstrated for SP.

KW - CALLA

KW - CD10

KW - E.C.3.4.24.11.

KW - Enkephalinase

KW - Enzyme kinetics

KW - HPLC

KW - Neutral metalloendopeptidase

KW - Peptidase inhibitors

KW - Peptidases

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UR - http://www.scopus.com/inward/citedby.url?scp=0025822334&partnerID=8YFLogxK

U2 - 10.1016/0196-9781(91)90102-U

DO - 10.1016/0196-9781(91)90102-U

M3 - Article

C2 - 1717955

AN - SCOPUS:0025822334

SN - 0196-9781

VL - 12

SP - 563

EP - 567

JO - Peptides

JF - Peptides

IS - 3

ER -

Catabolism of calcitonin gene-related peptide and substance P by neutral endopeptidase

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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