Cathepsin B and L activities in isolated osteoclasts

Barry R. Rifkin, Anthony T. Vernillo, Audrey P. Kleckner, Jeanine M. Auszmann, Linda R. Rosenberg, Morris Zimmerman

Research output: Contribution to journalArticlepeer-review

Abstract

Cathepsin B and L activities were examined with chicken osteoclasts isolated by sequential filtration and inhibitors were added to disaggregated rat osteoclasts on cortical bovine bone. Z-Phe-Phe-CHN2, a selective inhibitor of cathepsin L, at 1, 5, and 10 μM, inhibited bone resorption by rat osteoclasts 50, 85, and 100 per cent and, in chicken osteoclasts, cathepsin L activity was comparably inhibited. Cathepsin L in avian osteoclasts was also 25-fold higher than cathepsin B. Chicken osteoclasts treated with Z-Phe-Ala-CHN2, a generalized cysteine proteinase inhibitor, had both cathepsins inhibited to the same extent. Cathepsin L may play a key role in resorption.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume179
Issue number1
DOIs
StatePublished - Aug 30 1991

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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