TY - JOUR
T1 - Cathepsin B and L activities in isolated osteoclasts
AU - Rifkin, Barry R.
AU - Vernillo, Anthony T.
AU - Kleckner, Audrey P.
AU - Auszmann, Jeanine M.
AU - Rosenberg, Linda R.
AU - Zimmerman, Morris
N1 - Funding Information:
This work was supported by NIH grants DE-07788 and DE-09576 from the National Institute of Dental Research and presented, in part, at the American Association for Dental Research Meeting, San Francisco, California, March 1989.
PY - 1991/8/30
Y1 - 1991/8/30
N2 - Cathepsin B and L activities were examined with chicken osteoclasts isolated by sequential filtration and inhibitors were added to disaggregated rat osteoclasts on cortical bovine bone. Z-Phe-Phe-CHN2, a selective inhibitor of cathepsin L, at 1, 5, and 10 μM, inhibited bone resorption by rat osteoclasts 50, 85, and 100 per cent and, in chicken osteoclasts, cathepsin L activity was comparably inhibited. Cathepsin L in avian osteoclasts was also 25-fold higher than cathepsin B. Chicken osteoclasts treated with Z-Phe-Ala-CHN2, a generalized cysteine proteinase inhibitor, had both cathepsins inhibited to the same extent. Cathepsin L may play a key role in resorption.
AB - Cathepsin B and L activities were examined with chicken osteoclasts isolated by sequential filtration and inhibitors were added to disaggregated rat osteoclasts on cortical bovine bone. Z-Phe-Phe-CHN2, a selective inhibitor of cathepsin L, at 1, 5, and 10 μM, inhibited bone resorption by rat osteoclasts 50, 85, and 100 per cent and, in chicken osteoclasts, cathepsin L activity was comparably inhibited. Cathepsin L in avian osteoclasts was also 25-fold higher than cathepsin B. Chicken osteoclasts treated with Z-Phe-Ala-CHN2, a generalized cysteine proteinase inhibitor, had both cathepsins inhibited to the same extent. Cathepsin L may play a key role in resorption.
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U2 - 10.1016/0006-291X(91)91334-9
DO - 10.1016/0006-291X(91)91334-9
M3 - Article
C2 - 1883385
AN - SCOPUS:0026055964
SN - 0006-291X
VL - 179
SP - 63
EP - 69
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -