TY - JOUR
T1 - C/EBP is an immediate-early gene required for the consolidation of long-term facilitation in Aplysia
AU - Alberini, Cristina M.
AU - Ghirardl, Mirella
AU - Metz, Richard
AU - Kandel, Eric R.
N1 - Funding Information:
We thank Richard Axel, Dusan Bartsch, Thomas Jessell, and Steve McKnight for their comments on earlier revisions of this manuscript. This research was supported by the Howard Hughes Medical Institute (E. R. K.) and by a fellowship from the Human Frontier Science Program Organization to C. M. A., who is from Caltedra di Chimica Appli-cata alle Scienze Biomediche, University of Brescia, Brescia, Italy. We also thank Kaoru lnokuchi and Dietmar Kuhl, who provided the Aplysia cDNA and genomic libraries, Wayne Clemente for technical assistance, Sarah Mack and Chuck Lam for help with the figures, and Harriet Ayers and Andrew Krawetz for typing the manuscript
PY - 1994/3/25
Y1 - 1994/3/25
N2 - The consolidation of long-term memory requires protein and mRNA synthesis. A similar requirement has been demonstrated for learning-related synaptic plasticity in the gill-withdrawal reflex of Aplysia. The monosynaptic component of this reflex can be reconstituted in vitro, where it undergoes both short- and long-term increases in synaptic strength in response to serotonin (5-HT), a neurotransmitter released during behavioral sensitization, a simple form of learning. As with sensitization, the long-term synaptic modification is characterized by a brief consolidation period during which gene expression is required. We find that during this phase, the transcription factor Aplysia CCAAT enhancer-binding protein (ApC/EBP) is induced rapidly by 5-HT and by cAMP, even in the presence of protein synthesis inhibitors. Blocking the function of ApC/EBP blocks long-term facilitation selectively without affecting the short-term process. These data indicate that cAMP-inducible immediate-early genes have an essential role in the consolidation of stable long-term synaptic plasticity in Aplysia.
AB - The consolidation of long-term memory requires protein and mRNA synthesis. A similar requirement has been demonstrated for learning-related synaptic plasticity in the gill-withdrawal reflex of Aplysia. The monosynaptic component of this reflex can be reconstituted in vitro, where it undergoes both short- and long-term increases in synaptic strength in response to serotonin (5-HT), a neurotransmitter released during behavioral sensitization, a simple form of learning. As with sensitization, the long-term synaptic modification is characterized by a brief consolidation period during which gene expression is required. We find that during this phase, the transcription factor Aplysia CCAAT enhancer-binding protein (ApC/EBP) is induced rapidly by 5-HT and by cAMP, even in the presence of protein synthesis inhibitors. Blocking the function of ApC/EBP blocks long-term facilitation selectively without affecting the short-term process. These data indicate that cAMP-inducible immediate-early genes have an essential role in the consolidation of stable long-term synaptic plasticity in Aplysia.
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U2 - 10.1016/0092-8674(94)90386-7
DO - 10.1016/0092-8674(94)90386-7
M3 - Article
C2 - 8137425
AN - SCOPUS:0028280455
SN - 0092-8674
VL - 76
SP - 1099
EP - 1114
JO - Cell
JF - Cell
IS - 6
ER -