Cell and microsome mediated binding of 7, 12-dimethylbenz(a)anthracene to DNA studied by fluorescence spectroscopy

V. Ivanovic, N. E. Geacintov, A. M. Jeffrey, P. P. Fu, R. G. Harvey, I. B. Weinstein

Research output: Contribution to journalArticle

Abstract

Fluorescence spectra of DNA isolated from hamster embryo cells incubated with 7, 12-dimethylbenz(a)anthracene, or DNA modified in a microsomal system by reaction with this carcinogen or its 7-hydroxymethyl derivative, were compared to various model compounds. The spectra indicate that the DMBA derivative bound to DNA, in all 3 cases, has a 9, 10-dimethylanthracene-like chromophore. They also provide the first evidence of the similarity in structure of the DNA-bound products between 7, 12-dimethylbenz(a)anthracene and its 7-hydroxymethyl derivative. Our results are consistent with an activation mechanism that involves saturation of the 1, 2, 3, 4-ring positions.

Original languageEnglish (US)
Pages (from-to)131-140
Number of pages10
JournalCancer Letters
Volume4
Issue numberC
DOIs
StatePublished - 1978

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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