Cell free translation of chicken calcitonin messenger RNA

F. Lasmoles, C. Desplan, N. Segond, A. Jullienne, F. Treilhou-Lahille, G. Milhaud, M. S. Moukhtar

Research output: Contribution to journalArticlepeer-review

Abstract

The primary step of calcitonin biosynthesis was studied in a normal organ: chicken ultimobranchial gland, a tissue particularly rich in calcitonin secretory cells. Poly(A)-rich RNA was extracted and purified from ultimobranchial organs and translated in a reticulocyte lysate in the presence of labelled methionine. Polyacrylamide gel electrophoresis of specific immunoprecipitates revealed a major band of Mr 14 500 and a band of Mr 13 300. Thus, in chicken the precursor of calcitonin is a Mr 14500 polypeptide. The minor component of Mr 13 300 could represent limited processing by the reticulocyte lysate.

Original languageEnglish (US)
Pages (from-to)100-104
Number of pages5
JournalFEBS Letters
Volume157
Issue number1
DOIs
StatePublished - Jun 27 1983

Keywords

  • Calcitonin
  • Chicken
  • Messenger RNA
  • Radioimmunoassay
  • Translation
  • Ultimobranchial gland

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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