Abstract
The primary step of calcitonin biosynthesis was studied in a normal organ: chicken ultimobranchial gland, a tissue particularly rich in calcitonin secretory cells. Poly(A)-rich RNA was extracted and purified from ultimobranchial organs and translated in a reticulocyte lysate in the presence of labelled methionine. Polyacrylamide gel electrophoresis of specific immunoprecipitates revealed a major band of Mr 14 500 and a band of Mr 13 300. Thus, in chicken the precursor of calcitonin is a Mr 14500 polypeptide. The minor component of Mr 13 300 could represent limited processing by the reticulocyte lysate.
Original language | English (US) |
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Pages (from-to) | 100-104 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 157 |
Issue number | 1 |
DOIs | |
State | Published - Jun 27 1983 |
Keywords
- Calcitonin
- Chicken
- Messenger RNA
- Radioimmunoassay
- Translation
- Ultimobranchial gland
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology